A triplex real-time PCR assay for simultaneous and differential detection of Bordetella bronchiseptica, Mycoplasma cynos, and Mycoplasma canis in respiratory diseased dogs
Bordetella (B.) bronchiseptica, Mycoplasma (M.) cynos, and M. canis are the major bacterial pathogens that cause canine infectious respiratory disease complex (CIRDC). In this study, we developed a triplex real-time polymerase chain reaction (tqPCR) assay for the differential detection of these bacteria in a single reaction. The assay specifically amplified three bacterial genes with a detection limit of below 10 copies/reaction. The assay showed high repeatability and reproducibility, with coefficients of intra- and inter-assay variations of less than 1%. The diagnostic results of the assay u...
Korean J. Vet. Serv. 2023; 46(1): 15-27

Current Issue Volume 46, Number 1, March 2023


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About This Journal

The Korean Journal of Veterinary Service (Korean J. Vet. Serv.) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is published quarterly and is the official publication of the Korean J. Vet. Serv.. This journal is published in English or Korean by the KOSVES. It covers all the scientific and technological aspects of veterinary sciences in general, anatomy, physiology, biochemistry, pharmacology, microbiology, pathology, public health, parasitology, infectious diseases, clinical sciences, alternative veterinary medicine, animal welfare, food science of animal resources and other biomedical fields.

Korean Journal of
Veterinary Service

eISSN 2287-7630
pISSN 1225-6552
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