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Korean J. Vet. Serv. 2021; 44(4): 209-216

Published online December 30, 2021

https://doi.org/10.7853/kjvs.2021.44.4.209

© The Korean Socitety of Veterinary Service

대구지역 야생조류에서 분리된 대장균의 항생제 내성 조사

김경희ㆍ임현숙ㆍ이정우ㆍ박대현ㆍ양창렬ㆍ조재근*

대구광역시보건환경연구원

Received: October 11, 2021; Revised: December 27, 2021; Accepted: December 27, 2021

Antimicrobial resistance of Escherichia coli isolated from wild birds in Daegu

Kyung-Hee Kim , Hyun-Suk Lim , Jung-Woo Lee , Dae-Hyun Park , Chang-Ryoul Yang , Jae-Keun Cho *

Metropolitan Health & Enviornmental Research Institute, Daegu 42183, Korea

Correspondence to : Jae-Keun Cho
E-mail: thinking@korea.go.kr
https://orcid.org/0000-0003-3498-8101

Received: October 11, 2021; Revised: December 27, 2021; Accepted: December 27, 2021

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0). which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This study was aimed to investigate occurrence and the antimicrobial resistance of Escherichia coli isolates obtained from the feces of wild birds in Daegu. In total, 98 E. coli isolates (17.9%) were obtained from 547 fecal samples of wild birds. The E. coli carried by the birds showed a relatively high rate of antimicrobial resistance to tetracycline (27.6%) and ampicillin (21.4%). Drug resistance of the isolates to the others (penicillins, cephems, carbapenems, aminoglycosides, quinolones, sulfonamides and phenicols) resulted in the rates less than 20%, and all isolates were susceptible to imipenem, ciprofloxacin, cefotetAn, and amikacin. Approximately, 45% E. coli among the isolates were resistant to one or more drugs tested. The higher rate of tetracycline resistance led us to determine the prevalence of the tet genes (tetA, tetB, tetC, tetD and tetE) in the tetracycline-resistant E. coli isolates by using PCR. All isolates of the tetracycline-resistant E. coli contained at least one or more of these tet genes examined. The most prevalent one was tetA (59.3%), and followed by tetB (7.4%) when tested with the selected 5 tet genes. Except tetA and tetB , however, the remaining tet genes (tetC, tetD, and tetE) tested were not found in this study. Nine isolates among the tetracycline-resistant E. coli contained the two (tetA and tetB) determinants of tetracycline resistance, simultaneously.

Keywords Escherichia coli, Wild birds, Antimicrobial agents, Resistance gene

  1. Bonnedahl J, Järhult J, 2014. Antibiotic resisitance in wild birds. Ups J Med Sci 119(2): 113-116.
    Pubmed KoreaMed CrossRef
  2. Cho JK, Kim HD, Kwon SH, Kim JH, Jang SI, Park CK, Kim KS. 2014. Prevalence of antimicrobial reisstance and integrons in extended-spectrum B-lactamases producing Escherichia coli isolated from Nakdong and Gumbo river. Korean J Vet Res 37(1): 19-27.
    CrossRef
  3. Cho JK, Lee SM, Kim KS. 2008. Antimicrobial reisstance and distribution of tetracycline resistance determinants in Escherichia coli isolated from aquatic birds. Korean J Vet Res 48(3): 295-303.
  4. Cole D, Drum DJ, Stallknecht DE, White DG, Lee MD, Ayers S, Sobsey M, Maurer JJ. 2005. Free-living Canada geese and antimicrobial resistance. Emerg Infect Dis 11: 935-938.
    Pubmed KoreaMed CrossRef
  5. Fallacara DM, Monahan CM, Morishita TY, Wack RF. 2001. Fecal shedding and antimicrobial susceptibility of selected bacterial pathogens and a survey of intestinal parasites in free-living waterfowl. Avian Dis 45: 128-135.
    Pubmed CrossRef
  6. Huh MJ, Oh SS, Jang JS. 2013. Antimicrobial resistance and multi-drug resistance patterns of pathogenic bacteria isolated from food poisoning patienta in Incheon. Korean J Food & Nutr 26(1): 132-136.
    CrossRef
  7. Hwang IJ, Park DS, Chae HB, Kim ES, Yoon JH, Rajalingam N, Choi SV, Kim SR. 2020. Microbiological Quality and Antibiotic Susceptibility of E. coli Isolated from Agricultural Water in Gyeonggi and Gangwon Provinces. Korean J Environmental Agriculture 2020. 39(4): 343-351.
    CrossRef
  8. Kim HD, Park DH, Lee MR, Kim EJ, Cho JK. 2014. Characterization of cefotaxime-reisstant Escherichia coli isolated from wastewater treatment plant in Daegu. Korean J Vet Serv 37: 225-231.
    CrossRef
  9. Kim HR, Kim NH, Park HS, Kim YS, Lee JH. 2015. Antimicrobial resistance pattern and distribution of virulence-associated genes and serotypes of pathogenic Escherichia coli isolated from feces of wild birds in Seoul. Report SIHE 51: 170-179.
  10. Kim HT, Jung KT, Lee DS, Lee KW. 2009. Study on Antimicrobial resistance of Escherichia coli isolated from domestic beef on sale. Korean J Vet Serv 32(1): 93-102.
  11. Kim JH, Oh BY, Gong YW, Kim HY, Lee My, Koh YJ, Hwang KW, Lee JM. 2006. The trend fo anitimicribial resistance of Escherichia coli isolated from helathy volunteers of community and hospital patients in Incheon. Korean J Microbiol 42: 252-256.
  12. Kim MS, Kwon HM, Sung HW. 2009. Antibioticl reisstance pattern of avian pathogenic Escherichia coli isolated from chickens. Korean J Vet Res 49: 195-200.
  13. Kim YW, CC, Piotr G Jablonski, Lee SI. 2020. Detection of antibiotic-resistant Escherichia coli from feces of the Oriiental magpie nestlings. Korean J Omi 27(1): 3-9.
    CrossRef
  14. Kwak HJ, Lee WW, Kim JH, Chung KT, Woo BG, Lee GR, Lee DS. 2006. The antimicrobial susceptibility and plasmid profile of Escherichia coli from wild birds. Korean J Vet Res 29(1): 37-463.
  15. Lanz R, Kuhnert P, Boerlin P. 2003. Antimicrobial resistance and resistance gene determinants in clinical Escherichia coli from different animal species in Switzerland. Vet Microbiol 2: 73-84.
    CrossRef
  16. Lee HN, Yeo YG, Ahn SJ, Kim JT. 2020. Analysis fo the anti-microbial susceptibility of Clostridium isolated on clinical specimens from captive wild animals in Seoul Zoo. Korean J Vet Serv 43(1): 31-37.
    CrossRef
  17. Lee IH, Kim SK, Choi YH, Kim JS. 2006. Distribution and characteristics fo coliform bacteria in groundwater of Yeungnam. Korean J Microbiol 42(2): 95-102.
  18. Lee JK, Lee YK, Yuk YS, Kim GY. 2017. convergence study fo antimicrebial resistance of Escherichia coli isolated from Cheonho reservoir in Cheonan. J kor convergence society 8: 141-149.
  19. Lee YJ, Kim AR, Jung SC, Song SW, Kim JH. 2005. Antibiotic resistance pattren of Entericossus spp. Staphylococcus aureus isolated from chicken feces. Korean J Vet Res 45(2): 163-168.
  20. Lim SK, Nam HM, Moon DC, Jang GC, Jung SC. 2014. Antimicrobial resistance of Escherichia coli isolated from healthy animal during 2010-2012. Korean J Vet Res 54(3): 131-137.
    CrossRef
  21. Nataro JP, Boop CA, Fields PI, Kapper JB, Strockbine NA. 2007. 9. pp. 670-687. In: Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA(eds). Manual of Clinical Microbiology. ASM press, Washington DC.
  22. Normand EH, Gibson NR, Taylor DJ, Carmichael S. Reid SW 2000. Trends of antimicrobial resistance in bacterial isolates from a small animal referral hospital. Vet Rec 146: 151-155.
    Pubmed CrossRef
  23. Ong KH, Khor WC, Quek JY, Low ZX, Arivalan S Humaidi M, Chua C, Seow KL, Guo S, Tay M, Schlindt J, Ng LC, Aung KT. 2020. Occurrence and antimicrobial resistance traits of Escherichia coli from wild birds and rodents in Singapore. Int J Environ Res Public Health 17(15): 5606.
    Pubmed KoreaMed CrossRef
  24. Phillips I, Casewell M, Cox T, De Groot B, Friis C, Jones R, Nightingale C, Preston R, Waddell J. 2004. Does the use fo antibiotics in food animals pose a risk to human helath? A critical review of published data. J Antimicrob Chemother 53: 28-52.
    Pubmed CrossRef
  25. Sengel∅v G, Halling-Sorensen B, Aarestrup FM. 2003. Susceptibility of Escherichia coli and Enterococcus faecium isolated from pigs and broiler chickens to tetracycline degradation products and distribution of tetracycline resistance determinants in E. coli from food animals. Vet Microbiol 29: 91-101.
    CrossRef
  26. Shobrak MY, Abo-Amer A. 2014. Role of wild birds as carriers of multi-drug resistant Escherichia coli and Escheridhia vulneris. Braz J Microbiol 45(4): 1199-1209.
    Pubmed KoreaMed CrossRef
  27. Sung MS, Kim JH, Cho JK, Seol SY, Kim KS. 2008. Antimicrobial resistance and transfer of R plasmid of pathogenic Escherichia coli isolated from poultry in Korea. Korean J Vet Res 48(3): 275-285.
  28. Swayne DE, Boulianne M, Logue CM, McDougald LR, Nair V, Suarez DL. 2020. pp. 770-830. In: Swayne DE. Diseases of Poultry. 14th ed. Markono Print Media Pte Ltd, Singapore.
    CrossRef
  29. Tadesse DA, Zhao S, Tong E, Ayera S, Singh A, Bartholomew MJ, Mcdermott PF. 2012. Antimicrobial drug resistance in Escherichia coli from humans and food animals, United states, 1950-2002. Emerg Infect Dis 18(5): 741-749.
    Pubmed KoreaMed CrossRef
  30. Yoo YA, Kim MS, Kim KS, Park SH, Jung SK. 2010. Antimicrobial resistance and implicated genes of Escherichia coli isolated from commerical and cooked foods in Seoul. J FD Hygi Safety 25(3): 220-225.
  31. Yun SW, Kwon DY, Choi SK, Lee HS, Cho GJ. 2010. Characteristics and antimicrobial susceptibility of Escherichia coli isolated from horse. Korean J Vet Res 50(3): 231-237.

Article

Original Article

Korean J. Vet. Serv. 2021; 44(4): 209-216

Published online December 30, 2021 https://doi.org/10.7853/kjvs.2021.44.4.209

Copyright © The Korean Socitety of Veterinary Service.

대구지역 야생조류에서 분리된 대장균의 항생제 내성 조사

김경희ㆍ임현숙ㆍ이정우ㆍ박대현ㆍ양창렬ㆍ조재근*

대구광역시보건환경연구원

Received: October 11, 2021; Revised: December 27, 2021; Accepted: December 27, 2021

Antimicrobial resistance of Escherichia coli isolated from wild birds in Daegu

Kyung-Hee Kim , Hyun-Suk Lim , Jung-Woo Lee , Dae-Hyun Park , Chang-Ryoul Yang , Jae-Keun Cho *

Metropolitan Health & Enviornmental Research Institute, Daegu 42183, Korea

Correspondence to:Jae-Keun Cho
E-mail: thinking@korea.go.kr
https://orcid.org/0000-0003-3498-8101

Received: October 11, 2021; Revised: December 27, 2021; Accepted: December 27, 2021

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0). which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study was aimed to investigate occurrence and the antimicrobial resistance of Escherichia coli isolates obtained from the feces of wild birds in Daegu. In total, 98 E. coli isolates (17.9%) were obtained from 547 fecal samples of wild birds. The E. coli carried by the birds showed a relatively high rate of antimicrobial resistance to tetracycline (27.6%) and ampicillin (21.4%). Drug resistance of the isolates to the others (penicillins, cephems, carbapenems, aminoglycosides, quinolones, sulfonamides and phenicols) resulted in the rates less than 20%, and all isolates were susceptible to imipenem, ciprofloxacin, cefotetAn, and amikacin. Approximately, 45% E. coli among the isolates were resistant to one or more drugs tested. The higher rate of tetracycline resistance led us to determine the prevalence of the tet genes (tetA, tetB, tetC, tetD and tetE) in the tetracycline-resistant E. coli isolates by using PCR. All isolates of the tetracycline-resistant E. coli contained at least one or more of these tet genes examined. The most prevalent one was tetA (59.3%), and followed by tetB (7.4%) when tested with the selected 5 tet genes. Except tetA and tetB , however, the remaining tet genes (tetC, tetD, and tetE) tested were not found in this study. Nine isolates among the tetracycline-resistant E. coli contained the two (tetA and tetB) determinants of tetracycline resistance, simultaneously.

Keywords: Escherichia coli, Wild birds, Antimicrobial agents, Resistance gene

References

  1. Bonnedahl J, Järhult J, 2014. Antibiotic resisitance in wild birds. Ups J Med Sci 119(2): 113-116.
    Pubmed KoreaMed CrossRef
  2. Cho JK, Kim HD, Kwon SH, Kim JH, Jang SI, Park CK, Kim KS. 2014. Prevalence of antimicrobial reisstance and integrons in extended-spectrum B-lactamases producing Escherichia coli isolated from Nakdong and Gumbo river. Korean J Vet Res 37(1): 19-27.
    CrossRef
  3. Cho JK, Lee SM, Kim KS. 2008. Antimicrobial reisstance and distribution of tetracycline resistance determinants in Escherichia coli isolated from aquatic birds. Korean J Vet Res 48(3): 295-303.
  4. Cole D, Drum DJ, Stallknecht DE, White DG, Lee MD, Ayers S, Sobsey M, Maurer JJ. 2005. Free-living Canada geese and antimicrobial resistance. Emerg Infect Dis 11: 935-938.
    Pubmed KoreaMed CrossRef
  5. Fallacara DM, Monahan CM, Morishita TY, Wack RF. 2001. Fecal shedding and antimicrobial susceptibility of selected bacterial pathogens and a survey of intestinal parasites in free-living waterfowl. Avian Dis 45: 128-135.
    Pubmed CrossRef
  6. Huh MJ, Oh SS, Jang JS. 2013. Antimicrobial resistance and multi-drug resistance patterns of pathogenic bacteria isolated from food poisoning patienta in Incheon. Korean J Food & Nutr 26(1): 132-136.
    CrossRef
  7. Hwang IJ, Park DS, Chae HB, Kim ES, Yoon JH, Rajalingam N, Choi SV, Kim SR. 2020. Microbiological Quality and Antibiotic Susceptibility of E. coli Isolated from Agricultural Water in Gyeonggi and Gangwon Provinces. Korean J Environmental Agriculture 2020. 39(4): 343-351.
    CrossRef
  8. Kim HD, Park DH, Lee MR, Kim EJ, Cho JK. 2014. Characterization of cefotaxime-reisstant Escherichia coli isolated from wastewater treatment plant in Daegu. Korean J Vet Serv 37: 225-231.
    CrossRef
  9. Kim HR, Kim NH, Park HS, Kim YS, Lee JH. 2015. Antimicrobial resistance pattern and distribution of virulence-associated genes and serotypes of pathogenic Escherichia coli isolated from feces of wild birds in Seoul. Report SIHE 51: 170-179.
  10. Kim HT, Jung KT, Lee DS, Lee KW. 2009. Study on Antimicrobial resistance of Escherichia coli isolated from domestic beef on sale. Korean J Vet Serv 32(1): 93-102.
  11. Kim JH, Oh BY, Gong YW, Kim HY, Lee My, Koh YJ, Hwang KW, Lee JM. 2006. The trend fo anitimicribial resistance of Escherichia coli isolated from helathy volunteers of community and hospital patients in Incheon. Korean J Microbiol 42: 252-256.
  12. Kim MS, Kwon HM, Sung HW. 2009. Antibioticl reisstance pattern of avian pathogenic Escherichia coli isolated from chickens. Korean J Vet Res 49: 195-200.
  13. Kim YW, CC, Piotr G Jablonski, Lee SI. 2020. Detection of antibiotic-resistant Escherichia coli from feces of the Oriiental magpie nestlings. Korean J Omi 27(1): 3-9.
    CrossRef
  14. Kwak HJ, Lee WW, Kim JH, Chung KT, Woo BG, Lee GR, Lee DS. 2006. The antimicrobial susceptibility and plasmid profile of Escherichia coli from wild birds. Korean J Vet Res 29(1): 37-463.
  15. Lanz R, Kuhnert P, Boerlin P. 2003. Antimicrobial resistance and resistance gene determinants in clinical Escherichia coli from different animal species in Switzerland. Vet Microbiol 2: 73-84.
    CrossRef
  16. Lee HN, Yeo YG, Ahn SJ, Kim JT. 2020. Analysis fo the anti-microbial susceptibility of Clostridium isolated on clinical specimens from captive wild animals in Seoul Zoo. Korean J Vet Serv 43(1): 31-37.
    CrossRef
  17. Lee IH, Kim SK, Choi YH, Kim JS. 2006. Distribution and characteristics fo coliform bacteria in groundwater of Yeungnam. Korean J Microbiol 42(2): 95-102.
  18. Lee JK, Lee YK, Yuk YS, Kim GY. 2017. convergence study fo antimicrebial resistance of Escherichia coli isolated from Cheonho reservoir in Cheonan. J kor convergence society 8: 141-149.
  19. Lee YJ, Kim AR, Jung SC, Song SW, Kim JH. 2005. Antibiotic resistance pattren of Entericossus spp. Staphylococcus aureus isolated from chicken feces. Korean J Vet Res 45(2): 163-168.
  20. Lim SK, Nam HM, Moon DC, Jang GC, Jung SC. 2014. Antimicrobial resistance of Escherichia coli isolated from healthy animal during 2010-2012. Korean J Vet Res 54(3): 131-137.
    CrossRef
  21. Nataro JP, Boop CA, Fields PI, Kapper JB, Strockbine NA. 2007. 9. pp. 670-687. In: Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA(eds). Manual of Clinical Microbiology. ASM press, Washington DC.
  22. Normand EH, Gibson NR, Taylor DJ, Carmichael S. Reid SW 2000. Trends of antimicrobial resistance in bacterial isolates from a small animal referral hospital. Vet Rec 146: 151-155.
    Pubmed CrossRef
  23. Ong KH, Khor WC, Quek JY, Low ZX, Arivalan S Humaidi M, Chua C, Seow KL, Guo S, Tay M, Schlindt J, Ng LC, Aung KT. 2020. Occurrence and antimicrobial resistance traits of Escherichia coli from wild birds and rodents in Singapore. Int J Environ Res Public Health 17(15): 5606.
    Pubmed KoreaMed CrossRef
  24. Phillips I, Casewell M, Cox T, De Groot B, Friis C, Jones R, Nightingale C, Preston R, Waddell J. 2004. Does the use fo antibiotics in food animals pose a risk to human helath? A critical review of published data. J Antimicrob Chemother 53: 28-52.
    Pubmed CrossRef
  25. Sengel∅v G, Halling-Sorensen B, Aarestrup FM. 2003. Susceptibility of Escherichia coli and Enterococcus faecium isolated from pigs and broiler chickens to tetracycline degradation products and distribution of tetracycline resistance determinants in E. coli from food animals. Vet Microbiol 29: 91-101.
    CrossRef
  26. Shobrak MY, Abo-Amer A. 2014. Role of wild birds as carriers of multi-drug resistant Escherichia coli and Escheridhia vulneris. Braz J Microbiol 45(4): 1199-1209.
    Pubmed KoreaMed CrossRef
  27. Sung MS, Kim JH, Cho JK, Seol SY, Kim KS. 2008. Antimicrobial resistance and transfer of R plasmid of pathogenic Escherichia coli isolated from poultry in Korea. Korean J Vet Res 48(3): 275-285.
  28. Swayne DE, Boulianne M, Logue CM, McDougald LR, Nair V, Suarez DL. 2020. pp. 770-830. In: Swayne DE. Diseases of Poultry. 14th ed. Markono Print Media Pte Ltd, Singapore.
    CrossRef
  29. Tadesse DA, Zhao S, Tong E, Ayera S, Singh A, Bartholomew MJ, Mcdermott PF. 2012. Antimicrobial drug resistance in Escherichia coli from humans and food animals, United states, 1950-2002. Emerg Infect Dis 18(5): 741-749.
    Pubmed KoreaMed CrossRef
  30. Yoo YA, Kim MS, Kim KS, Park SH, Jung SK. 2010. Antimicrobial resistance and implicated genes of Escherichia coli isolated from commerical and cooked foods in Seoul. J FD Hygi Safety 25(3): 220-225.
  31. Yun SW, Kwon DY, Choi SK, Lee HS, Cho GJ. 2010. Characteristics and antimicrobial susceptibility of Escherichia coli isolated from horse. Korean J Vet Res 50(3): 231-237.
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