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Korean J. Vet. Serv. 2020; 43(4): 261-265

Published online December 30, 2020

https://doi.org/10.7853/kjvs.2020.43.4.261

© The Korean Socitety of Veterinary Service

Determination of cyromazine in commercial insecticides using HPLC-DAD

Kim, Young-Wook;Han, Bok Hee;Kang, Young Eun;Rhee, Chae Hong;Seo, Sang-Ji;Kim, Soohee;Jeong, Wooseog;Her, Moon;

Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;

Correspondence to : Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency

Received: December 6, 2020; Revised: December 18, 2020; Accepted: December 20, 2020

Abstract

Each commercial cyromazine insecticide has different HPLC conditions. The aim of this study was to establish a standardized chromatographic method for the determination of cyromazine in commercial insecticides. The separation was achieved on two C18 columns - Waters® Bondapak C (4×300 nm i.d., 10 ㎛) and X bridge (4.6×250 nm i.d., 5 ㎛) using a mobile phase composed of water/methanol/ethanolamine (76:24:0.1, v/v), with UV detection at wavelengths 230 nm and 254 nm. A total of six commercial cyromazine insecticides were analyzed. In this study, the optimal high-performance liquid chromatography conditions for the analysis of cyromazine were as follows: a mobile phase of water/methanol/ethanolamine (76:24:0.1, v/v) at a flow rate of 1.0 mL/min and a detection wavelength of 230 nm using a X bridge C18 column (4.6×250 nm i.d., 5 ㎛) at a column temperature of 25℃. The calibration curve was linear in the concentration range of 5~50 ㎍/mL, with a correlation coefficient of 0.99995. The cyromazine detection limit was 0.2 ㎍/mL, and the limit of quantification was 0.59 ㎍/mL. The percentage recovery ranged from 99.8% to 101.0% for cyromazine, and the relative standard deviation was not over 2.0%. The cyromazine concentration ranged from 92.7% to 109.4% and was within the acceptable range (90~120%) for the percent of the labeled amount. This method was found to be suitable for determining cyromazine in commercial insecticides.

Keywords Cyromazine,Insecticide,HPLC,Validation,

Article

Research Article

Korean J. Vet. Serv. 2020; 43(4): 261-265

Published online December 30, 2020 https://doi.org/10.7853/kjvs.2020.43.4.261

Copyright © The Korean Socitety of Veterinary Service.

Determination of cyromazine in commercial insecticides using HPLC-DAD

Kim, Young-Wook;Han, Bok Hee;Kang, Young Eun;Rhee, Chae Hong;Seo, Sang-Ji;Kim, Soohee;Jeong, Wooseog;Her, Moon;

Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency;

Correspondence to:Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency

Received: December 6, 2020; Revised: December 18, 2020; Accepted: December 20, 2020

Abstract

Each commercial cyromazine insecticide has different HPLC conditions. The aim of this study was to establish a standardized chromatographic method for the determination of cyromazine in commercial insecticides. The separation was achieved on two C18 columns - Waters® Bondapak C (4×300 nm i.d., 10 ㎛) and X bridge (4.6×250 nm i.d., 5 ㎛) using a mobile phase composed of water/methanol/ethanolamine (76:24:0.1, v/v), with UV detection at wavelengths 230 nm and 254 nm. A total of six commercial cyromazine insecticides were analyzed. In this study, the optimal high-performance liquid chromatography conditions for the analysis of cyromazine were as follows: a mobile phase of water/methanol/ethanolamine (76:24:0.1, v/v) at a flow rate of 1.0 mL/min and a detection wavelength of 230 nm using a X bridge C18 column (4.6×250 nm i.d., 5 ㎛) at a column temperature of 25℃. The calibration curve was linear in the concentration range of 5~50 ㎍/mL, with a correlation coefficient of 0.99995. The cyromazine detection limit was 0.2 ㎍/mL, and the limit of quantification was 0.59 ㎍/mL. The percentage recovery ranged from 99.8% to 101.0% for cyromazine, and the relative standard deviation was not over 2.0%. The cyromazine concentration ranged from 92.7% to 109.4% and was within the acceptable range (90~120%) for the percent of the labeled amount. This method was found to be suitable for determining cyromazine in commercial insecticides.

Keywords: Cyromazine,Insecticide,HPLC,Validation,

KJVS
Dec 30, 2022 Vol.45 No.4, pp. 249~342

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