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Korean J. Vet. Serv. 2016; 39(4): 259-266

Published online December 30, 2016

https://doi.org/10.7853/kjvs.2016.39.4.259

© The Korean Socitety of Veterinary Service

충남지역 돼지유행성설사 바이러스 Spike 유전자 염기서열 및 계통분석

박효선;육심용;전동민;이진주;신창호;

충청남도가축위생연구소 역학조사과;충청남도가축위생연구소 역학조사과;충청남도가축위생연구소 역학조사과;충청남도가축위생연구소 역학조사과;충청남도가축위생연구소 역학조사과;

Received: December 13, 2016; Revised: December 26, 2016; Accepted: December 26, 2016

Genetic sequence and phylogenetic analysis of spike genes of Porcine epidemic diarrhea virus (PEDV) in Chung-Nam area

Park, Hyo-Seon;Yook, Sim-Yong;Jeon, Dong-Min;Lee, Jin-Ju;Shin, Chang-Ho;

Veterinary Epidemiology Division, Chungnam Veterinary Research Institute;Veterinary Epidemiology Division, Chungnam Veterinary Research Institute;Veterinary Epidemiology Division, Chungnam Veterinary Research Institute;Veterinary Epidemiology Division, Chungnam Veterinary Research Institute;Veterinary Epidemiology Division, Chungnam Veterinary Research Institute;

Correspondence to : 충청남도가축위생연구소 역학조사과(Veterinary Epidemiology Division, Chungnam Veterinary Research Institute)

Received: December 13, 2016; Revised: December 26, 2016; Accepted: December 26, 2016

Abstract

Porcine epidemic diarrhea virus (PEDV) causes an acute and lethal watery diarrhea in piglets that is great economic losses to the swine country worldwide. The spike (S) glycoprotein is an important determinant for PEDV biological properties. In the present study, we determined the full-length S gene sequences of five Chung-nam PEDV field isolates collected in 2016. The S gene was amplified by RT-PCR, purificated, sequenced, analyzed and then compared with published sequences of other PEDV strains. 5 field strains share 98.5%~99.9% homologies with each other at the nucleotide sequence level and 96.7%~99.9% homologies with each other at the amino acids sequence level. Most field strains have nucleotide insertions, deletions and mutation regions, and show lower homologies (93.1~93.8%) with classical and vaccine strains, however higher homologies (99.1%~99.5%) with US PEDV isolates in 2013. By phylogenetic tree analysis based on nucleotide sequence, five PEDV field isolates were clustered into Genogroup 2b but differ genetically from the vaccine strains (SM-98 and DR-13).

Keywords PEDV,S gene,PCR,Phylogenetic analysis,

Article

Research Article

Korean J. Vet. Serv. 2016; 39(4): 259-266

Published online December 30, 2016 https://doi.org/10.7853/kjvs.2016.39.4.259

Copyright © The Korean Socitety of Veterinary Service.

충남지역 돼지유행성설사 바이러스 Spike 유전자 염기서열 및 계통분석

박효선;육심용;전동민;이진주;신창호;

충청남도가축위생연구소 역학조사과;충청남도가축위생연구소 역학조사과;충청남도가축위생연구소 역학조사과;충청남도가축위생연구소 역학조사과;충청남도가축위생연구소 역학조사과;

Received: December 13, 2016; Revised: December 26, 2016; Accepted: December 26, 2016

Genetic sequence and phylogenetic analysis of spike genes of Porcine epidemic diarrhea virus (PEDV) in Chung-Nam area

Park, Hyo-Seon;Yook, Sim-Yong;Jeon, Dong-Min;Lee, Jin-Ju;Shin, Chang-Ho;

Veterinary Epidemiology Division, Chungnam Veterinary Research Institute;Veterinary Epidemiology Division, Chungnam Veterinary Research Institute;Veterinary Epidemiology Division, Chungnam Veterinary Research Institute;Veterinary Epidemiology Division, Chungnam Veterinary Research Institute;Veterinary Epidemiology Division, Chungnam Veterinary Research Institute;

Correspondence to:충청남도가축위생연구소 역학조사과(Veterinary Epidemiology Division, Chungnam Veterinary Research Institute)

Received: December 13, 2016; Revised: December 26, 2016; Accepted: December 26, 2016

Abstract

Porcine epidemic diarrhea virus (PEDV) causes an acute and lethal watery diarrhea in piglets that is great economic losses to the swine country worldwide. The spike (S) glycoprotein is an important determinant for PEDV biological properties. In the present study, we determined the full-length S gene sequences of five Chung-nam PEDV field isolates collected in 2016. The S gene was amplified by RT-PCR, purificated, sequenced, analyzed and then compared with published sequences of other PEDV strains. 5 field strains share 98.5%~99.9% homologies with each other at the nucleotide sequence level and 96.7%~99.9% homologies with each other at the amino acids sequence level. Most field strains have nucleotide insertions, deletions and mutation regions, and show lower homologies (93.1~93.8%) with classical and vaccine strains, however higher homologies (99.1%~99.5%) with US PEDV isolates in 2013. By phylogenetic tree analysis based on nucleotide sequence, five PEDV field isolates were clustered into Genogroup 2b but differ genetically from the vaccine strains (SM-98 and DR-13).

Keywords: PEDV,S gene,PCR,Phylogenetic analysis,

KJVS
Mar 30, 2024 Vol.47 No.1, pp. 1~7

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Korean Journal of
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