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Korean J. Vet. Serv. 2015; 38(2): 141-144
Published online June 30, 2015
https://doi.org/10.7853/kjvs.2015.38.2.141
© The Korean Socitety of Veterinary Service
The first virus isolation and partial characterization of equine herpesvirus-4 in a horse, South Korea
Correspondence to : Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency
An equine herpesvirus-4 (EHV-4) was isolated in nasal swabs collected in a horse showing respiratory clinical signs. Equine dermis cells inoculated with the sample were observed with characteristic viral cytopathic effects after 3 days of postinoculation and the infected cells exhibited bright intracelluar fluorescence by indirect immunofluorescence assay. At the nucleotide level, the partial glycoprotein B gene of the Korean EHV-4 isolate (K001) had 99.9% identity to 1942 strain (GenBank No. M26171). To author's knowledge, the report describes the first isolation and partial characterization of EHV-4 in Korea. The virus can be used for further study of EHV-4.
Keywords Equine herpesvirus-4,Korea,Virus isolation,Immunofluorescence assay,Glycoprotein B,
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Research Article
Korean J. Vet. Serv. 2015; 38(2): 141-144
Published online June 30, 2015 https://doi.org/10.7853/kjvs.2015.38.2.141
Copyright © The Korean Socitety of Veterinary Service.
The first virus isolation and partial characterization of equine herpesvirus-4 in a horse, South Korea
Choi, Eun-Jin;Lee, Hyun-Kyoung;Lee, Kyoung-Hyun;So, Byoung-Jae;Song, Jae-Young;Do, Jae-Chul;Yang, Seon-Joo;Lee, Hyun-Chul;Yang, Young-Jin;
Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency;Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency;Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency;Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency;Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency;Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency;Equine Hospital, Korea Racing Authority;Equine Hospital, Korea Racing Authority;Equine Hospital, Korea Racing Authority;
Correspondence to:Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency
Abstract
An equine herpesvirus-4 (EHV-4) was isolated in nasal swabs collected in a horse showing respiratory clinical signs. Equine dermis cells inoculated with the sample were observed with characteristic viral cytopathic effects after 3 days of postinoculation and the infected cells exhibited bright intracelluar fluorescence by indirect immunofluorescence assay. At the nucleotide level, the partial glycoprotein B gene of the Korean EHV-4 isolate (K001) had 99.9% identity to 1942 strain (GenBank No. M26171). To author's knowledge, the report describes the first isolation and partial characterization of EHV-4 in Korea. The virus can be used for further study of EHV-4.
Keywords: Equine herpesvirus-4,Korea,Virus isolation,Immunofluorescence assay,Glycoprotein B,
