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Korean J. Vet. Serv. 2012; 35(4): 295-305

Published online December 30, 2012

https://doi.org/10.7853/kjvs.2012.35.4.295

© The Korean Socitety of Veterinary Service

유산양 유즙으로부터 분리된 세균의 분포 및 항균제 감수성 검사

김혜라;정지영;김선득;박준영;조인영;신성식;손창호;오기석;허태영;정영훈;최창용;서국현;

전남축산위생사업소;전남축산위생사업소;전남대학교 수의과대학;전남대학교 수의과대학;전남대학교 수의과대학;전남대학교 수의과대학;전남대학교 수의과대학;전남대학교 수의과대학;농촌진흥청 축산과학원;농촌진흥청 축산과학원;농촌진흥청 축산과학원;전남대학교 수의과대학;

Received: August 27, 2012; Revised: November 14, 2012; Accepted: November 19, 2012

Isolation and antimicrobial susceptibility of microorganisms from milk samples of dairy goat

Kim, Hye-Ra;Jung, Ji-Young;Kim, Seon-Deuk;Park, Jun-Young;Cho, In-Young;Shin, Sung-Shik;Son, Chang-Ho;Ok, Ki-Seok;Hur, Tai-Young;Jung, Young-Hun;Choi, Chang-Yong;Suh, Guk-Hyun;

Jeollanamdo Livestock Sanitation Office;Jeollanamdo Livestock Sanitation Office;College of Veterinary Medicine, Chonnam National University;College of Veterinary Medicine, Chonnam National University;College of Veterinary Medicine, Chonnam National University;College of Veterinary Medicine, Chonnam National University;College of Veterinary Medicine, Chonnam National University;College of Veterinary Medicine, Chonnam National University;National Institute of Animal Science, RDA;National Institute of Animal Science, RDA;National Institute of Animal Science, RDA;College of Veterinary Medicine, Chonnam National University;

Correspondence to : 전남대학교 수의과대학(College of Veterinary Medicine, Chonnam National University)

Received: August 27, 2012; Revised: November 14, 2012; Accepted: November 19, 2012

Abstract

The aim of this study was to isolate microorganisms from half milk samples of dairy goats by California mastitis test (CMT) during the lactation period and to further investigate the susceptibility of isolated organisms to antimicrobial drugs. From a total of 235 half milk samples with CMT scores of 2 or above from 366 dairy goats distributed throughout Jeonnam province, microorganisms were isolated from 198 (83.5%) samples either singly (99.0%) or in combination (1.0%). The most prevalent microorganism was the coagulase-negative Staphylococcus spp., (44.4%, n=88) followed by Staphylococcus aureus (24.2%, n=48), Escherichia coli (11.1%, n=22) and Streptococcus spp. (7.6%, n=15). Isolated bacteria also included Bacillus spp. (2.5%, n=5), Pseudomonas spp. (2.5%, n=5), Micrococcus spp. (1.5%, n=3), Corynebacterium spp. (1.5%, n=3), Enterococcus facium (1.0%, n=2), Morganella morganii (0.5%, n=1) and Streptococcus agalactiae (0.5%, n=1). During the summer season, a high prevalence of all microorganisms were observed in which Staphylococcus spp. (30.8%), Escherichia coli (8.6%), and Streptococcus spp. (5.6%) were among the most prevalent bacteria isolated. Staphylococcus spp. was also shown to be high in the winter (21.7%). In most samples, the presence of bacterial pathogens in goat milk led to the increase in the total somatic cell count (SCC). Most of the half milk samples of dairy goats with bacterial contamination showed SCC of ${geq}1{ imes}10^6cells/ml$ (90.4%). Minor pathogens (11.4%) were more detected from milk samples with SCC of < $1{ imes}10^6cells/ml$ than major pathogens (4.1%), while the major pathogens tended to be higher from samples with SCC of ${geq}3{ imes}10^6cells/ml$. Susceptibility of these bacteria to 12 antimicrobial agents was tested by the Kirby-Bauer disc diffusion method. Results indicated that more than 90% of bacteria isolated from CMT 2+ dairy goat half milk samples were susceptible to trimethoprim/sulfamethoxazole, amoxicillin/clavulanic, enrofloxacin and cephalothin while they were resistant to tetracycline (44.7%).

Keywords Dairy goat,Mastitis,SCC,Bacteria,Antimicrobial susceptibility,

Article

Research Article

Korean J. Vet. Serv. 2012; 35(4): 295-305

Published online December 30, 2012 https://doi.org/10.7853/kjvs.2012.35.4.295

Copyright © The Korean Socitety of Veterinary Service.

유산양 유즙으로부터 분리된 세균의 분포 및 항균제 감수성 검사

김혜라;정지영;김선득;박준영;조인영;신성식;손창호;오기석;허태영;정영훈;최창용;서국현;

전남축산위생사업소;전남축산위생사업소;전남대학교 수의과대학;전남대학교 수의과대학;전남대학교 수의과대학;전남대학교 수의과대학;전남대학교 수의과대학;전남대학교 수의과대학;농촌진흥청 축산과학원;농촌진흥청 축산과학원;농촌진흥청 축산과학원;전남대학교 수의과대학;

Received: August 27, 2012; Revised: November 14, 2012; Accepted: November 19, 2012

Isolation and antimicrobial susceptibility of microorganisms from milk samples of dairy goat

Kim, Hye-Ra;Jung, Ji-Young;Kim, Seon-Deuk;Park, Jun-Young;Cho, In-Young;Shin, Sung-Shik;Son, Chang-Ho;Ok, Ki-Seok;Hur, Tai-Young;Jung, Young-Hun;Choi, Chang-Yong;Suh, Guk-Hyun;

Jeollanamdo Livestock Sanitation Office;Jeollanamdo Livestock Sanitation Office;College of Veterinary Medicine, Chonnam National University;College of Veterinary Medicine, Chonnam National University;College of Veterinary Medicine, Chonnam National University;College of Veterinary Medicine, Chonnam National University;College of Veterinary Medicine, Chonnam National University;College of Veterinary Medicine, Chonnam National University;National Institute of Animal Science, RDA;National Institute of Animal Science, RDA;National Institute of Animal Science, RDA;College of Veterinary Medicine, Chonnam National University;

Correspondence to:전남대학교 수의과대학(College of Veterinary Medicine, Chonnam National University)

Received: August 27, 2012; Revised: November 14, 2012; Accepted: November 19, 2012

Abstract

The aim of this study was to isolate microorganisms from half milk samples of dairy goats by California mastitis test (CMT) during the lactation period and to further investigate the susceptibility of isolated organisms to antimicrobial drugs. From a total of 235 half milk samples with CMT scores of 2 or above from 366 dairy goats distributed throughout Jeonnam province, microorganisms were isolated from 198 (83.5%) samples either singly (99.0%) or in combination (1.0%). The most prevalent microorganism was the coagulase-negative Staphylococcus spp., (44.4%, n=88) followed by Staphylococcus aureus (24.2%, n=48), Escherichia coli (11.1%, n=22) and Streptococcus spp. (7.6%, n=15). Isolated bacteria also included Bacillus spp. (2.5%, n=5), Pseudomonas spp. (2.5%, n=5), Micrococcus spp. (1.5%, n=3), Corynebacterium spp. (1.5%, n=3), Enterococcus facium (1.0%, n=2), Morganella morganii (0.5%, n=1) and Streptococcus agalactiae (0.5%, n=1). During the summer season, a high prevalence of all microorganisms were observed in which Staphylococcus spp. (30.8%), Escherichia coli (8.6%), and Streptococcus spp. (5.6%) were among the most prevalent bacteria isolated. Staphylococcus spp. was also shown to be high in the winter (21.7%). In most samples, the presence of bacterial pathogens in goat milk led to the increase in the total somatic cell count (SCC). Most of the half milk samples of dairy goats with bacterial contamination showed SCC of ${geq}1{ imes}10^6cells/ml$ (90.4%). Minor pathogens (11.4%) were more detected from milk samples with SCC of < $1{ imes}10^6cells/ml$ than major pathogens (4.1%), while the major pathogens tended to be higher from samples with SCC of ${geq}3{ imes}10^6cells/ml$. Susceptibility of these bacteria to 12 antimicrobial agents was tested by the Kirby-Bauer disc diffusion method. Results indicated that more than 90% of bacteria isolated from CMT 2+ dairy goat half milk samples were susceptible to trimethoprim/sulfamethoxazole, amoxicillin/clavulanic, enrofloxacin and cephalothin while they were resistant to tetracycline (44.7%).

Keywords: Dairy goat,Mastitis,SCC,Bacteria,Antimicrobial susceptibility,

KJVS
Sep 30, 2024 Vol.47 No.3, pp. 115~191

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