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Korean J. Vet. Serv. 2010; 33(2): 105-111
Published online June 30, 2010
© The Korean Socitety of Veterinary Service
광견병바이러스에 대한 단크론항체 생산 및 특성
이승철;윤영심;송윤경;우계형;진영화;강신영;
중앙백신연구소;충북대학교 수의과대학;국립수의과학검역원;국립수의과학검역원;국립수의과학검역원;충북대학교 수의과대학;
Production and characterization of monoclonal antibodies against rabies virus
Correspondence to : 충북대학교 수의과대학(College of Veterinary Medicine, Chungbuk National University)
Rabies virus which belongs to the genus Lyssavirus of the family Rhabdoviridae is known as a highly neurotropic virus and causes fatal encephalitis accompanied by severe neurological symptoms in almost all mammals, including humans. In this study, monoclonal antibodies (MAbs) against rabies virus were produced, characterized and applications of MAbs as diagnostic reagents were assessed Spleen and inguinal lymph node cells from Balb/c mouse immunized with purified rabies virus were fused with SP2/O myeloma cells using polyethylene glycol (PEG) and hybridoma cells producing rabies virus-specific MAbs were screened by an indirect fluorescent antibody test. A total of ten MAbs were produced against rabies virus. The protein specificity and neutralizing activity of MAbs were determined by Western blot analysis and fluorescent antibody virus neutralization test, respectively. As a result, two MAbs, 5G3 and 6H4 had specificity for nucleoprotein (N protein) and two other MAbs, 5B1 and 5C1 had neutralizing activity for rabies virus. Some MAbs recognized the rabies virus-infected bovine brain stem cells by immunohistochemistry (IHC) assay. In conclusion, it was confirmed that MAbs produced in this study were rabies virusspecific and could be used as reliable diagnostic reagents for the detection of rabies virus.
Keywords Rabies virus,Monoclonal antibody,Immunohistochemistry,
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Research Article
Korean J. Vet. Serv. 2010; 33(2): 105-111
Published online June 30, 2010
Copyright © The Korean Socitety of Veterinary Service.
광견병바이러스에 대한 단크론항체 생산 및 특성
이승철;윤영심;송윤경;우계형;진영화;강신영;
중앙백신연구소;충북대학교 수의과대학;국립수의과학검역원;국립수의과학검역원;국립수의과학검역원;충북대학교 수의과대학;
Production and characterization of monoclonal antibodies against rabies virus
Lee, Seung-Chul;Yoon, Young-Sim;Song, Yun-Kyung;Woo, Gye-Hyeong;Jean, Young-Hwa;Kang, Shien-Young;
Choongang Vaccine Laboratories;College of Veterinary Medicine, Chungbuk National University;National Veterinary Research and Quarantine Service;National Veterinary Research and Quarantine Service;National Veterinary Research and Quarantine Service;College of Veterinary Medicine, Chungbuk National University;
Correspondence to:충북대학교 수의과대학(College of Veterinary Medicine, Chungbuk National University)
Abstract
Rabies virus which belongs to the genus Lyssavirus of the family Rhabdoviridae is known as a highly neurotropic virus and causes fatal encephalitis accompanied by severe neurological symptoms in almost all mammals, including humans. In this study, monoclonal antibodies (MAbs) against rabies virus were produced, characterized and applications of MAbs as diagnostic reagents were assessed Spleen and inguinal lymph node cells from Balb/c mouse immunized with purified rabies virus were fused with SP2/O myeloma cells using polyethylene glycol (PEG) and hybridoma cells producing rabies virus-specific MAbs were screened by an indirect fluorescent antibody test. A total of ten MAbs were produced against rabies virus. The protein specificity and neutralizing activity of MAbs were determined by Western blot analysis and fluorescent antibody virus neutralization test, respectively. As a result, two MAbs, 5G3 and 6H4 had specificity for nucleoprotein (N protein) and two other MAbs, 5B1 and 5C1 had neutralizing activity for rabies virus. Some MAbs recognized the rabies virus-infected bovine brain stem cells by immunohistochemistry (IHC) assay. In conclusion, it was confirmed that MAbs produced in this study were rabies virusspecific and could be used as reliable diagnostic reagents for the detection of rabies virus.
Keywords: Rabies virus,Monoclonal antibody,Immunohistochemistry,
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