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Korean J. Vet. Serv. 2002; 25(4): 347-355

Published online September 1, 2002

© The Korean Socitety of Veterinary Service

Apoptosis in experimentally infected chicks with avian infectious bronchitis

Song, Sun-Kyong;Lee, Jong-Hoon;Park, Yeon-Cheol;Shin, Yong-Uk;Park, Il-Gue;

Puyeo Branch, Chungnam Livestock and Veterinary Research Institute;Puyeo Branch, Chungnam Livestock and Veterinary Research Institute;Puyeo Branch, Chungnam Livestock and Veterinary Research Institute;Puyeo Branch, Chungnam Livestock and Veterinary Research Institute;Puyeo Branch, Chungnam Livestock and Veterinary Research Institute;

Correspondence to : Puyeo Branch, Chungnam Livestock and Veterinary Research Institute

Abstract

This experiment was performed to investigate apoptosis during undergoing pathogenesis of avian infectious bronchitis virus(IBV)-infected chicks. Sixteen days old chicks were infected with IBV, Massachusetts-41 strain(M-41, 10$^4$-10$^{5}$ EID$^{50}$ ) experimentally, they were autopsied to remove trachea, lung, kidney and cloacal bursa at 6hr, 12hr, 1 day, 3 day and 7 day post infection(PI) respectively for H-E and TUNEL staining. Grossly, mild serous, catarrhal exudate was observed in the trachea, nasal passages and sinuses nasal from 4 day PI. The cloacal bursa was swollen from 3 day PI. Histopathologically, the trachea was seen mild cellular infiltration, edema of the mucosa and submucosa, vascular congestion and mild hyperplasia of the epithelium from 6 hr PI and the changes were seen a little more severely on 7 day PI. It was observed that the cloacal bursa was getting more and more hyperplasia through the experiment. The nuclei degeneration were shown in the kidney on 7 day PI. No specific changes were seen in the lung. In TUNEL analysis, apoptotic cells showed sharp increasing at 12 hr PI and reaching a maximum on 1 day PI in the trachea, lung, kidney and cloacal bursa. And then apoptotic cells decreased gradually returning to a level of the control by 7 day PI in all the removed organs.

Keywords IBV,Apoptosis,Histopathologic,TUNEL analysis,

Article

Research Article

Korean J. Vet. Serv. 2002; 25(4): 347-355

Published online September 1, 2002

Copyright © The Korean Socitety of Veterinary Service.

Apoptosis in experimentally infected chicks with avian infectious bronchitis

Song, Sun-Kyong;Lee, Jong-Hoon;Park, Yeon-Cheol;Shin, Yong-Uk;Park, Il-Gue;

Puyeo Branch, Chungnam Livestock and Veterinary Research Institute;Puyeo Branch, Chungnam Livestock and Veterinary Research Institute;Puyeo Branch, Chungnam Livestock and Veterinary Research Institute;Puyeo Branch, Chungnam Livestock and Veterinary Research Institute;Puyeo Branch, Chungnam Livestock and Veterinary Research Institute;

Correspondence to:Puyeo Branch, Chungnam Livestock and Veterinary Research Institute

Abstract

This experiment was performed to investigate apoptosis during undergoing pathogenesis of avian infectious bronchitis virus(IBV)-infected chicks. Sixteen days old chicks were infected with IBV, Massachusetts-41 strain(M-41, 10$^4$-10$^{5}$ EID$^{50}$ ) experimentally, they were autopsied to remove trachea, lung, kidney and cloacal bursa at 6hr, 12hr, 1 day, 3 day and 7 day post infection(PI) respectively for H-E and TUNEL staining. Grossly, mild serous, catarrhal exudate was observed in the trachea, nasal passages and sinuses nasal from 4 day PI. The cloacal bursa was swollen from 3 day PI. Histopathologically, the trachea was seen mild cellular infiltration, edema of the mucosa and submucosa, vascular congestion and mild hyperplasia of the epithelium from 6 hr PI and the changes were seen a little more severely on 7 day PI. It was observed that the cloacal bursa was getting more and more hyperplasia through the experiment. The nuclei degeneration were shown in the kidney on 7 day PI. No specific changes were seen in the lung. In TUNEL analysis, apoptotic cells showed sharp increasing at 12 hr PI and reaching a maximum on 1 day PI in the trachea, lung, kidney and cloacal bursa. And then apoptotic cells decreased gradually returning to a level of the control by 7 day PI in all the removed organs.

Keywords: IBV,Apoptosis,Histopathologic,TUNEL analysis,

KJVS
Dec 30, 2024 Vol.47 No.4, pp. 193~317

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Korean Journal of
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