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Korean J. Vet. Serv. 2021; 44(4): 283-290

Published online December 30, 2021

https://doi.org/10.7853/kjvs.2021.44.4.283

© The Korean Socitety of Veterinary Service

가축 전염병 관리를 위한 돼지 사체의 화학적 처리 방법

오연수1ㆍ윤석훈2ㆍ탁동섭3ㆍ조호성4*

강원대학교 수의과대학 및 동물의학종합연구소1, (주)에스티아이2, 전북대학교 인수공통전염병연구소3, 전북대학교 수의과대학 및 생체안전성연구소4

Received: December 25, 2021; Accepted: December 27, 2021

Validation of method for chemical treatment of pig carcasses for the control of livestock infectious diseases

Yeonsu Oh 1, Suk Hoon Yoon 2, Dong-Seob Tark 3, Ho-Seong Cho 4*

1College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea
2STI Co., Ltd., Daegu 42921, Korea
3Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Korea
4College of Veterinary Medicine and Bio-Safety Research Institute, Jeonbuk National University, Iksan 54596, Korea

Correspondence to : Ho-Seong Cho
E-mail: hscho@jbnu.ac.kr
https://orcid.org/0000-0001-7443-167X

Received: December 25, 2021; Accepted: December 27, 2021

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0). which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This is a study on the improvement of the chemical treatment method of the livestock carcass treatment newly introduced in the livestock infectious disease prevention method in order to improve the problems of the existing burial-centered carcass treatment method when a livestock infectious disease occurs. It was conducted to establish detailed treatment standards for the chemical treatment method of pig carcasses based on the results of proof of the absence of infectious diseases in pigs. After inoculating pig carcasses with 10 pathogens (6 viruses [FMDV, ASFV, CSFV, PCV2, PRRSV, PEDV] and 4 bacteria [Lawsonia intracellularis, Clostridium perfringens type C, E. coli, Salmonella Typhimurium]) It was treated at 90℃ for 5 hours in a potassium hydroxide (KOH) liquid solution corresponding to 15% of the body weight. This method liquefies all cadaveric components and inactivates all inoculated pathogens. Based on these results, it was possible to prove that chemical treatment of pig carcasses is effective in killing pathogens and is a safe method without the risk of disease transmission. Although there are problems to be solved in the processing and operation of the chemical treatment products of livestock carcasses, the chemical treatment method of livestock carcasses can be suggested as an alternative to the current domestic burial-centered livestock carcass treatment method, preventing environmental pollution, and contributing to public health.

Keywords Alkaline hydrolysis, Biosecurity, Disaster-type animal disease, Infectious animal disease, Pig

  1. Animal and Plant Quarantine Agency, Korea Animal Health Integrated System. 2021 (cited 2021 Dec. 1).
    Available from: http://kahis.go.kr.
  2. Cho HS, Kim GH. 2012. Needs of biosecurity and protocols for the environmental management of carcasses burial. J Korean Soc Water Environ. 28(2): 305-312.
  3. Cho HS. 2012. Detection of foot-and-mouth disease virus and coxsakievirus in the soil and leachate of modeled carcass burial site. Korean J Vet Serv. 35(4): 255-261.
    CrossRef
  4. Cho HS. 2020. Establishment of selection criteria and biosecurity manual for breeding complex of cloven-hoofed animals. Policy research service report of Ministry of Agriculture, Food and Rural Affairs. pp. 31-36.
  5. Franke-Whittle IH, Insam H. 2013. Treatment alternatives of slaughterhouse wastes, and their effect on the inactivation of different pathogens: a review. Crit Rev Microbiol. 39(2): 139-151.
    Pubmed KoreaMed CrossRef
  6. Gwyther CL, Williams AP, Golyshin PN, Edwards-Jones G, Jones DL. 2011. The environmental and biosecurity characteristics of livestock carcass disposal methods: A review. Waste Manag. 31: 767-778.
    Pubmed CrossRef
  7. Kaye G, Weber P, Evans A, Venezia R. 1998. Efficacy of Alkaline Hydrolysis as an Alternative Method for Treatment and Disposal of Infectious Animal Waste. Contemp Top Lab Anim Sci. 37(3): 43-46.
  8. Kim BN, Cho HS, Cha Y, Park JK, Kim G, Kim YH, Min J. 2016. Effect of Corynebacterium glutamicum on Livestock Material Burial Treatment. J Microbiol Biotechnol. 26(8): 1404-1408.
    Pubmed CrossRef
  9. Lemire KA, Rodriguez YY, McIntosh MT. 2016. Alkaline hydrolysis to remove potentially infectious viral RNA contaminants from DNA. Virol J. 13: 88.
    Pubmed KoreaMed CrossRef
  10. Ministry of Agriculture, Forestry and Rural Affairs. 2021. Act on the prevention of contagious animal doseases. Available from https://www.law.go.kr/LSW/eng/engLsSc.do?menuId=2§ion=lawNm&query=ACT+ON+THE+PREVENTION+OF+CONTAGIOUS+ANIMA.
  11. Murphy RGL, Scanga JA, Powers BE, Pilon JL, Vercauteren KC, Nash PB, Smith GC, Belk KE. 2009. Alkaline hydrolysis of mouse-adapted scrapie for inactivation and disposal of prion-positive material. J Anim Sci. 87(5): 1787-1793.
    Pubmed CrossRef
  12. Seo YJ, Seo DC, Choi IW, Kang SW, Lee SG, Sung HH, Kim TS, Kim HG, Park SH, Kang SJ, Cho JS. 2012. Selection of optimal degradation agents for hydrolysis of animal cadavers. Korean J Soil Sci. Fert. 45(2): 241-247.
    CrossRef

Article

Original Article

Korean J. Vet. Serv. 2021; 44(4): 283-290

Published online December 30, 2021 https://doi.org/10.7853/kjvs.2021.44.4.283

Copyright © The Korean Socitety of Veterinary Service.

가축 전염병 관리를 위한 돼지 사체의 화학적 처리 방법

오연수1ㆍ윤석훈2ㆍ탁동섭3ㆍ조호성4*

강원대학교 수의과대학 및 동물의학종합연구소1, (주)에스티아이2, 전북대학교 인수공통전염병연구소3, 전북대학교 수의과대학 및 생체안전성연구소4

Received: December 25, 2021; Accepted: December 27, 2021

Validation of method for chemical treatment of pig carcasses for the control of livestock infectious diseases

Yeonsu Oh 1, Suk Hoon Yoon 2, Dong-Seob Tark 3, Ho-Seong Cho 4*

1College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea
2STI Co., Ltd., Daegu 42921, Korea
3Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Korea
4College of Veterinary Medicine and Bio-Safety Research Institute, Jeonbuk National University, Iksan 54596, Korea

Correspondence to:Ho-Seong Cho
E-mail: hscho@jbnu.ac.kr
https://orcid.org/0000-0001-7443-167X

Received: December 25, 2021; Accepted: December 27, 2021

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0). which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This is a study on the improvement of the chemical treatment method of the livestock carcass treatment newly introduced in the livestock infectious disease prevention method in order to improve the problems of the existing burial-centered carcass treatment method when a livestock infectious disease occurs. It was conducted to establish detailed treatment standards for the chemical treatment method of pig carcasses based on the results of proof of the absence of infectious diseases in pigs. After inoculating pig carcasses with 10 pathogens (6 viruses [FMDV, ASFV, CSFV, PCV2, PRRSV, PEDV] and 4 bacteria [Lawsonia intracellularis, Clostridium perfringens type C, E. coli, Salmonella Typhimurium]) It was treated at 90℃ for 5 hours in a potassium hydroxide (KOH) liquid solution corresponding to 15% of the body weight. This method liquefies all cadaveric components and inactivates all inoculated pathogens. Based on these results, it was possible to prove that chemical treatment of pig carcasses is effective in killing pathogens and is a safe method without the risk of disease transmission. Although there are problems to be solved in the processing and operation of the chemical treatment products of livestock carcasses, the chemical treatment method of livestock carcasses can be suggested as an alternative to the current domestic burial-centered livestock carcass treatment method, preventing environmental pollution, and contributing to public health.

Keywords: Alkaline hydrolysis, Biosecurity, Disaster-type animal disease, Infectious animal disease, Pig

References

  1. Animal and Plant Quarantine Agency, Korea Animal Health Integrated System. 2021 (cited 2021 Dec. 1). Available from: http://kahis.go.kr.
  2. Cho HS, Kim GH. 2012. Needs of biosecurity and protocols for the environmental management of carcasses burial. J Korean Soc Water Environ. 28(2): 305-312.
  3. Cho HS. 2012. Detection of foot-and-mouth disease virus and coxsakievirus in the soil and leachate of modeled carcass burial site. Korean J Vet Serv. 35(4): 255-261.
    CrossRef
  4. Cho HS. 2020. Establishment of selection criteria and biosecurity manual for breeding complex of cloven-hoofed animals. Policy research service report of Ministry of Agriculture, Food and Rural Affairs. pp. 31-36.
  5. Franke-Whittle IH, Insam H. 2013. Treatment alternatives of slaughterhouse wastes, and their effect on the inactivation of different pathogens: a review. Crit Rev Microbiol. 39(2): 139-151.
    Pubmed KoreaMed CrossRef
  6. Gwyther CL, Williams AP, Golyshin PN, Edwards-Jones G, Jones DL. 2011. The environmental and biosecurity characteristics of livestock carcass disposal methods: A review. Waste Manag. 31: 767-778.
    Pubmed CrossRef
  7. Kaye G, Weber P, Evans A, Venezia R. 1998. Efficacy of Alkaline Hydrolysis as an Alternative Method for Treatment and Disposal of Infectious Animal Waste. Contemp Top Lab Anim Sci. 37(3): 43-46.
  8. Kim BN, Cho HS, Cha Y, Park JK, Kim G, Kim YH, Min J. 2016. Effect of Corynebacterium glutamicum on Livestock Material Burial Treatment. J Microbiol Biotechnol. 26(8): 1404-1408.
    Pubmed CrossRef
  9. Lemire KA, Rodriguez YY, McIntosh MT. 2016. Alkaline hydrolysis to remove potentially infectious viral RNA contaminants from DNA. Virol J. 13: 88.
    Pubmed KoreaMed CrossRef
  10. Ministry of Agriculture, Forestry and Rural Affairs. 2021. Act on the prevention of contagious animal doseases. Available from https://www.law.go.kr/LSW/eng/engLsSc.do?menuId=2§ion=lawNm&query=ACT+ON+THE+PREVENTION+OF+CONTAGIOUS+ANIMA.
  11. Murphy RGL, Scanga JA, Powers BE, Pilon JL, Vercauteren KC, Nash PB, Smith GC, Belk KE. 2009. Alkaline hydrolysis of mouse-adapted scrapie for inactivation and disposal of prion-positive material. J Anim Sci. 87(5): 1787-1793.
    Pubmed CrossRef
  12. Seo YJ, Seo DC, Choi IW, Kang SW, Lee SG, Sung HH, Kim TS, Kim HG, Park SH, Kang SJ, Cho JS. 2012. Selection of optimal degradation agents for hydrolysis of animal cadavers. Korean J Soil Sci. Fert. 45(2): 241-247.
    CrossRef
KJVS
Sep 30, 2022 Vol.45 No.3, pp. 145~248

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