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Korean J. Vet. Serv. 2004; 27(1): 81-87
Published online March 1, 2004
© The Korean Socitety of Veterinary Service
산란계 밀집지역에서 혈구응집억제반응과 효소면역측정법을 이용한 가금인플루엔자 혈중항체가의 비교 조사
이정원;엄성심;이성재;서이원;서석열;정동석;송희종;
전라북도축산진흥연구소;전라북도축산진흥연구소;전라북도축산진흥연구소;전라북도축산진흥연구소;전라북도축산진흥연구소;전라북도축산진흥연구소;전북대학교 생체안전성연구소;
Comparative study on avian influenza virus antibody titer by hemagglutination inhibition test and enzyme-linked immunosorbent assay in the mass zone layer
Correspondence to : 전라북도축산진흥연구소
This study was conducted to investigate the similarity between hemagglutination inhibition (HI) test and enzyme-linked immunosorbent assay(ELISA), the HI titer and mean ratio S/P ratio) of avian influenza virus. To perform this study, the 1,457 sera of layers 21 farms in May, July and September, respectively. As a result of HI test, positive rates were 480 to 422 (92.1%) in May, 494 to 394(79.8%) in July and 483 to 402(83.2%) in September, and the mean antibody titer were 4.6, 4.3, 4.0 to 0.3 decreased, respectively. The positive rates by ELISA, 480 to 475(99.0%) in May, 494 to 485(98.2%) in July, 483 to 472(97.7%) in September, and the mean S/P ratio were 2.319, 2.557 and 2.380, respectively. The result of HI test and ELISA positive 480 to 422(92.1%), 475(99.0%), 494 to 394(79.8%), 485(98.2%) and 483 to 402(83.2%), 472(97.7%). Therefore, ELISA was shown more sensitive compare the HI titers.
Keywords Avian influenza virus,Layer,HI test,ELISA,
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Research Article
Korean J. Vet. Serv. 2004; 27(1): 81-87
Published online March 1, 2004
Copyright © The Korean Socitety of Veterinary Service.
산란계 밀집지역에서 혈구응집억제반응과 효소면역측정법을 이용한 가금인플루엔자 혈중항체가의 비교 조사
이정원;엄성심;이성재;서이원;서석열;정동석;송희종;
전라북도축산진흥연구소;전라북도축산진흥연구소;전라북도축산진흥연구소;전라북도축산진흥연구소;전라북도축산진흥연구소;전라북도축산진흥연구소;전북대학교 생체안전성연구소;
Comparative study on avian influenza virus antibody titer by hemagglutination inhibition test and enzyme-linked immunosorbent assay in the mass zone layer
이정원;엄성심;이성재;서이원;서석열;정동석;송희종;
Correspondence to:전라북도축산진흥연구소
Abstract
This study was conducted to investigate the similarity between hemagglutination inhibition (HI) test and enzyme-linked immunosorbent assay(ELISA), the HI titer and mean ratio S/P ratio) of avian influenza virus. To perform this study, the 1,457 sera of layers 21 farms in May, July and September, respectively. As a result of HI test, positive rates were 480 to 422 (92.1%) in May, 494 to 394(79.8%) in July and 483 to 402(83.2%) in September, and the mean antibody titer were 4.6, 4.3, 4.0 to 0.3 decreased, respectively. The positive rates by ELISA, 480 to 475(99.0%) in May, 494 to 485(98.2%) in July, 483 to 472(97.7%) in September, and the mean S/P ratio were 2.319, 2.557 and 2.380, respectively. The result of HI test and ELISA positive 480 to 422(92.1%), 475(99.0%), 494 to 394(79.8%), 485(98.2%) and 483 to 402(83.2%), 472(97.7%). Therefore, ELISA was shown more sensitive compare the HI titers.
Keywords: Avian influenza virus,Layer,HI test,ELISA,
