Top
Search 닫기

Research Article

Split Viewer

Korean J. Vet. Serv. 2014; 37(1): 19-27

Published online March 30, 2014

https://doi.org/10.7853/kjvs.2014.37.1.19

© The Korean Socitety of Veterinary Service

낙동강과 금호강에서 분리된 광범위 베타 락탐 분해효소 생성 Escherichia coli 내 항균제 내성 및 integrons의 분포

조재근;김환득;권순효;김진현;장성일;박최규;김기석;

대구광역시보건환경연구원;대구광역시보건환경연구원;대구광역시보건환경연구원;경상북도가축위생시험소;대구광역시보건환경연구원;경북대학교 수의과대학;경북대학교 수의과대학;

Received: January 2, 2014; Revised: February 10, 2014; Accepted: March 4, 2014

Prevalence of antimicrobial resistance and integrons in extended-spectrum β-lactamases producing Escherichia coli isolated from Nakdong and Gumho river

Cho, Jae-Keun;Kim, Hwan-Deuk;Kwon, Soon-Hyo;Kim, Jin-Hyun;Jang, Sung-Il;Park, Choi-Kyu;Kim, Ki-Seuk;

Metropolitan Health & Environmental Research Institute;Metropolitan Health & Environmental Research Institute;Metropolitan Health & Environmental Research Institute;Gyeongbuk Veterinary Service Laboratory;Metropolitan Health & Environmental Research Institute;College of Veterinary Medicine, Kyungpook National University;College of Veterinary Medicine, Kyungpook National University;

Correspondence to : 경북대학교 수의과대학(College of Veterinary Medicine, Kyungpook National University)

Received: January 2, 2014; Revised: February 10, 2014; Accepted: March 4, 2014

Abstract

This study was conducted to investigate the antimicrobial resistance, presence of ${eta}$-lactamase genes and integrons in 83 ESBL-producing Escherichia coli isolated from Nakdong river and Geumho river in Daegu. Among the ${eta}$-lactam antimicrobials, all isolates were resistant to ampicillin, cephalothin, cefamandole and cefotaxime, followed by piperacillin (98.8%), ampicillin/sulbactam (86.7%), aztreonam (60.2%) and cefepime (59.0%), whereas resistance to piperacillin/tazobacram, ticarcillin/clavulanic acid and cefoxitin was less than 30%. Many of the ESBL-producing Escherichia coli were also resistant to non-${eta}$-lactams antimicrobials such as nalidixic acid (83.1%), sulfonamides (72.3%), ciprofloxacin (62.7%) and gentamicin (38.6%). All isolates showed resistance to seven or more antimicrobial agents. The most frequently detected gene was $bla_{TEM+CTX-M}$ (49.4%), followed by $bla_{CTX-M}$ (27.7%), $bla_{TEM}$ (6.0%) and $bla_{OXA}$ (1.2%). But $bla_{SHV}$ was not found. Class 1 integrons were found in 61.4% (51 isolates) of isolates, however, class 2 and 3 integrons were not detected. The results showed water from Nakdong river and Geumho river is contaminated with ESBL-producing E. coli isolates. These results suggest the need for further investigation of antibiotic resistant bacteria to prevent public health impacts in the water environment.

Keywords River water,E. coli,Antimicrobial resistance,ESBL,Integron,

Article

Research Article

Korean J. Vet. Serv. 2014; 37(1): 19-27

Published online March 30, 2014 https://doi.org/10.7853/kjvs.2014.37.1.19

Copyright © The Korean Socitety of Veterinary Service.

낙동강과 금호강에서 분리된 광범위 베타 락탐 분해효소 생성 Escherichia coli 내 항균제 내성 및 integrons의 분포

조재근;김환득;권순효;김진현;장성일;박최규;김기석;

대구광역시보건환경연구원;대구광역시보건환경연구원;대구광역시보건환경연구원;경상북도가축위생시험소;대구광역시보건환경연구원;경북대학교 수의과대학;경북대학교 수의과대학;

Received: January 2, 2014; Revised: February 10, 2014; Accepted: March 4, 2014

Prevalence of antimicrobial resistance and integrons in extended-spectrum β-lactamases producing Escherichia coli isolated from Nakdong and Gumho river

Cho, Jae-Keun;Kim, Hwan-Deuk;Kwon, Soon-Hyo;Kim, Jin-Hyun;Jang, Sung-Il;Park, Choi-Kyu;Kim, Ki-Seuk;

Metropolitan Health & Environmental Research Institute;Metropolitan Health & Environmental Research Institute;Metropolitan Health & Environmental Research Institute;Gyeongbuk Veterinary Service Laboratory;Metropolitan Health & Environmental Research Institute;College of Veterinary Medicine, Kyungpook National University;College of Veterinary Medicine, Kyungpook National University;

Correspondence to:경북대학교 수의과대학(College of Veterinary Medicine, Kyungpook National University)

Received: January 2, 2014; Revised: February 10, 2014; Accepted: March 4, 2014

Abstract

This study was conducted to investigate the antimicrobial resistance, presence of ${eta}$-lactamase genes and integrons in 83 ESBL-producing Escherichia coli isolated from Nakdong river and Geumho river in Daegu. Among the ${eta}$-lactam antimicrobials, all isolates were resistant to ampicillin, cephalothin, cefamandole and cefotaxime, followed by piperacillin (98.8%), ampicillin/sulbactam (86.7%), aztreonam (60.2%) and cefepime (59.0%), whereas resistance to piperacillin/tazobacram, ticarcillin/clavulanic acid and cefoxitin was less than 30%. Many of the ESBL-producing Escherichia coli were also resistant to non-${eta}$-lactams antimicrobials such as nalidixic acid (83.1%), sulfonamides (72.3%), ciprofloxacin (62.7%) and gentamicin (38.6%). All isolates showed resistance to seven or more antimicrobial agents. The most frequently detected gene was $bla_{TEM+CTX-M}$ (49.4%), followed by $bla_{CTX-M}$ (27.7%), $bla_{TEM}$ (6.0%) and $bla_{OXA}$ (1.2%). But $bla_{SHV}$ was not found. Class 1 integrons were found in 61.4% (51 isolates) of isolates, however, class 2 and 3 integrons were not detected. The results showed water from Nakdong river and Geumho river is contaminated with ESBL-producing E. coli isolates. These results suggest the need for further investigation of antibiotic resistant bacteria to prevent public health impacts in the water environment.

Keywords: River water,E. coli,Antimicrobial resistance,ESBL,Integron,

KJVS
Mar 30, 2024 Vol.47 No.1, pp. 1~7

Stats or Metrics

Share this article on

  • line

Korean Journal of
Veterinary Service

eISSN 2287-7630
pISSN 1225-6552
qr-code Download