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Korean J. Vet. Serv. 2021; 44(2): 93-102

Published online June 30, 2021

https://doi.org/10.7853/kjvs.2021.44.2.93

© The Korean Socitety of Veterinary Service

대전 주택가 산책로 진드기의 인수공통전염병 병원체 감염실태 조사

한소영;성선혜;서진우;김종호;이석주;유상식;

대전광역시보건환경연구원 동물위생시험소;대전광역시보건환경연구원 동물위생시험소;대전광역시보건환경연구원 동물위생시험소;대전광역시보건환경연구원 동물위생시험소;대전광역시보건환경연구원 동물위생시험소;대전광역시보건환경연구원 동물위생시험소;

Received: April 26, 2021; Revised: June 19, 2021; Accepted: June 19, 2021

Isolation and identification of tick-borne pathogens in hard ticks collected in Daejeon

Han, So-young;Sung, Sun-hye;Seo, Jin-woo;Kim, Jong-ho;Lee, Seok-ju;Yoo, Sang-sik;

Daejeon Institute of Health and Environment Division of Animal Health;Daejeon Institute of Health and Environment Division of Animal Health;Daejeon Institute of Health and Environment Division of Animal Health;Daejeon Institute of Health and Environment Division of Animal Health;Daejeon Institute of Health and Environment Division of Animal Health;Daejeon Institute of Health and Environment Division of Animal Health;

Correspondence to : 대전광역시보건환경연구원 동물위생시험소(Daejeon Institute of Health and Environment Division of Animal Health)

Received: April 26, 2021; Revised: June 19, 2021; Accepted: June 19, 2021

In this study, a total of 9,449 hard ticks were collected once a month from April to October 2020 from a neighborhood park in Daejeon by flagging & dragging method and CO2 manned trap method. The collected ticks were classified according to the Yamagutsi search table using a stereoscopic microscope and molecular biological analysis of four pathogens (SFTSV, Anaplasma spp., Ehrlichia spp., Borrellia spp.). As a result of the study, Haemaphysalis longicornis were collected the most in all areas of the five boroughs at a rate of 82 to 96 percent, while adults were collected the most in May to July, nymphs were collected the most in April to June, and larvae from August to October at a rate of 78 percent to 98 percent. In pathogens, three cases of SFTSV were detected, showing a minimum infection rate (MIR) of 0.46%, while Anaplasma spp. and Ehrlichia spp. were detected one each, with 0.15% and Borrelia spp. with a minimum infection rate of 0.46%. The detected SFTSV showed 99.9% homogeneity with the KF781490 detected in Cheongwon-gun, Chungbuk Province, Anaplasma spp. showed 99.0% homogeneity with JN990105 detected in China, and Erhlichia spp. showed 98.9% genetic similarity with U96436 separated from the U.S. In this study, the distribution status and pathogen infection rate of the hard ticks in the Daejeon area are analyzed and provided as basic data for the prevention of the hard tick-borne infectious disease.

Keywords Hard tick,SFTS,Anaplasma,Borrelia,Phylogenetic tree,

  1. 국가가축방역통합시스템. 법정가축전염병 발생현황.
    Available from: http://www.kahis.go.kr/home/lkntscrinfo/selectLkntsOccrrncList.do. Cited [2020.06.29.].
  2. 기상청, 기상자료제공포털(http://data.kma.go.kr), 대전, 2020.
  3. 서재화, 임재원, 강동호, 김흥철. 질병 매개 참진드기류의 분류와 발생현황(I). 국립생물자원관. 2015.
  4. 질병관리본부, 감염병포털, 진드기 매개감염병 관리지침. 발간등록번호 11-3152159-000823-10, pp. 66-78
  5. Wodecka et al, 2007. Significance of red deer in the ecology of Borrelia burgdorferi sensu lato, Wiad Parazytol 53: 231-237.
  6. CDC, 2017. DPDx. https://www.cdc.gov/dpdx/ticks/index.html (accessed on 31 December, 2017).
  7. Chae JB, Kim TH, Jung JH, 2017. Prevalence of severe fever with thrombocytopenia syndrome virus among ticks surveyed at Mt. Gwanak, Korea, Korean J Vet 57(3): 169- 173.
    CrossRef
  8. Chae MB, Lee JY, Kwak YG, Park SH, Lim HJ, Park SW, Chung MH, Kim MK, Kang JS, 2002. Prevalence of antibodies to Bartonella henselae and BArtonella quintana in Korean patients with Lymphadenopathy. Korean J Infect Dis 34(5): 305-310.
  9. Chong ST, Kim HC, Lee IY, Kollars TM Jr, Sancho AR, Sames WJ, Chae JS, Klein TA, 2013. Seasonal distribution of ticks in four habitats near the demilitarized zone, Gyeong gi-do(Province). Republic of Korea, J Parasitol 51: 319-325.
    Pubmed KoreaMed CrossRef
  10. Gai ZT, Zhang Y, Liang MF, Jin C, Zhang S, Zhu CB Bian PF, 2012. Clinical progress and risk factors for death in severe fever with thrombocytopenia syndrome patients. J Infect Dis 206: 1095-1102.
    Pubmed CrossRef
  11. Han SY, Lee SJ, Joung EY, Choi KS, Woo IS, Kim JH, Yoo SS, 2020. Investigation into Prevalence of zoonotic infections of companion animals in Daejeon, The report of Daejeon Institute of Health and Environment.
  12. Hwang C.Y., 2011, Ehrlichia and Borrelia spp. Infection in German shephered Dogs in Korea, J Vet Clin 28(2): 204-211.
  13. Ismail N, Bloch KC. McBride JW. 2010. Human Ehrlichiosis and An aplasmosis. Clin Lab Med 30: 261-292.
    Pubmed KoreaMed CrossRef
  14. Kang JG, Kim HC, 2013. Molecular detection of Anaplasma, Bartonella, and Borrelia species in Ticks collected from migratory birds from Hong-do island, Republic of Korea, Vector-Borned and Zoonotic Diseases 13: 1-11.
    Pubmed CrossRef
  15. KCDC, 2014. Survey on anaplasmosis and ehrlichiosis from SFTS suspicious patients in South Korea. Public Health Weekly Report, Korea Centers for Disease Control and Prevention (KCDC) 7: 979-982.
  16. KCDC, Disease Web Statistics System. Korea Centers for Disease Control and Prevention (KCDC), 2020, https://www.cdc.go.kr/npt/biz/npp/nppMain.do/ (accessed 29 June 2020).
  17. Kim CM, Kim JK, Yi YH, Lee MJ, Cho MR, Devendra H, Terry A. Klein, Kim HC, Song JW, Chong ST, Monica L. O’Guinn, 2005. Detection of Bartonella species from ticks, mites and small mammals in Korea. J Vet Sci 6(4): 327-334.
    Pubmed CrossRef
  18. Kim HC, Han SH, Chong ST, Klein TA, Choi CY, Nam HY, Chae HY, Lee H, 2011. Ticks collected from Selected Mammalian Hosts surveyed in the Republic of Korea during2008-2009. 2011. Korean J Parasitol 49: 331-335.
    Pubmed KoreaMed CrossRef
  19. Kim JB, Song HW, Park SU, Park SW, Ahn JH, Eom YB, Kim YM, 1998. Detection of Borrelia burgdorferi and Ehrlichiosis agent in ticks collected in Korea using polymerase chain reaction. Korean J Biomed 4(2): 113-120.
  20. Kim KH, Oh MD, 2014. Severe Fever with Thrombocytopenia Syndrome. Korean journal of Medicine 86: 271-274.
    CrossRef
  21. Kim KH, Yi J, Kim G. et al, 2012. Severe fever with thrombocytopenia syndrome. South Korea. Emerg Infect Dis 19: 1892-1894.
    CrossRef
  22. Kim KH, Yi J, Oh WS, Kim NH, Choi SJ, Choe PG, Kim NJ, Lee JK, Oh MD. 2014. Human graulocytic anaplasmosis, South Korea, Emerg Infec Dis 20: 1708-1711.
    Pubmed KoreaMed CrossRef
  23. Lee SO, Na DK, Kim CM, Lee YH, Cho YH, Park JH, Lee JH, Eo SK, Terry A. Klein, Chae JS, 2005. Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis bassed on 16S rRNA gene. J Vet Sci 6(2): 151-155.
    Pubmed CrossRef
  24. Moon S, Gwack J, Hwang KJ, Kwon D, Kim S, Noh Y, Roh J, Shin E-h, Jeong K, Seok W, 2013. Autochthonous lyme borreliosis in humans and ticks in Korea. Osong Public Health Res Perspect 4: 52-56.
    Pubmed KoreaMed CrossRef
  25. Noh BE, 2019. Geographical distribution and relative abundance of hard ticks in the Republic of Korea. Graduate School, Kosin University, doctoral dissertation. http://www.riss.kr/link?id=T15342418 (accessed 29 June 2020)
  26. Noh KW, Kim YJ, Ryu NS, Park JH, Kim YR, Na MS, 2017, Molecular detection for tick-borne pathogens in hard ticks collected in Jeollabukdo. 2017. The report of Jeollabukdo Animal health Division.
  27. Picken RN, Strle F, Picken MM, Ruzic-Sabljic E, Maraspin V, Lotric-Furlan S, Cimperman J., 1998. Identification of three species of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto, B. garinii, and B. afzelii) among isolates from acrodermatitis chronica atrophicans lesions. J Invest Dermatol 110: 211-214.
    Pubmed CrossRef
  28. Qin XR, Han FJ, Luo LM, et al. 2018. Anaplasma species detected in Haemaphysalis longicornis tick from China. Tick and Tick-borne Disease 9: 840-843.
    Pubmed CrossRef
  29. Sachar DS. 2000. Ehrlichia chaffeensis infection in an active duty soldier stationed in Korea. Medical Surveillance Monthly Report 6: 9-11.
  30. Shin S. 2013. Severe fever with thrombocytopenia syndrome virus. J Korean Vet Med Assoc 49: 359-364.
  31. Shinje Moon, Jin Gwack, Kyu Jam Hwang, Donghyuk Kwon, Suyeon Kim, Yoonatae Noh, Jongyul Roh, E-hyun Shin, Kungjin Jeong, Wonseok seok, Seung-Ki Youn, 2013. Autochthonous Lyme Borreliosis in Humans and Ticks in Korea. Osong Public Health Res Perspect 4(1): 52-56.
    Pubmed KoreaMed CrossRef
  32. Steere AC. 1989. Lyme disease. N. Engl. J. Med. 321: 586-596.
    Pubmed CrossRef
  33. Suh JH, Lim JW, Kang DH, Kim HC. 2015. Taxonomy and Occurrences of Disease Mediating Ticks. NIBR NO. 201501110.
  34. Takahashi T, Maeda K, Suzuki T, Ishido A, Shigeoka T, Tominaga T, 2014. The first identification and retrospective study of severe fever with thrombocytopenia syndrome in Japan. J Infect Dis 209: 816-827.
    Pubmed KoreaMed CrossRef
  35. Yu XJ, Liang MF, Zhang SY, 2011. Fever with thrombocytopenia associated with a novel bunyavirus in China. N Engl J Med 364: 1523-1532.
  36. Yun SM, Lee YJ, Choi WY, Kim HC, Chong ST, Chang KS, Jordan M. Coburn, Terry A. Klein, Lee WJ, 2016. Molecular detection of severe fever with thrombocytopenia syndromeand tick-borne encephalitis viruses in ixodid ticks collected from vegetation. Ticks and Tick- borne Diseases 7: 970-978.
    Pubmed CrossRef

Article

Research Article

Korean J. Vet. Serv. 2021; 44(2): 93-102

Published online June 30, 2021 https://doi.org/10.7853/kjvs.2021.44.2.93

Copyright © The Korean Socitety of Veterinary Service.

대전 주택가 산책로 진드기의 인수공통전염병 병원체 감염실태 조사

한소영;성선혜;서진우;김종호;이석주;유상식;

대전광역시보건환경연구원 동물위생시험소;대전광역시보건환경연구원 동물위생시험소;대전광역시보건환경연구원 동물위생시험소;대전광역시보건환경연구원 동물위생시험소;대전광역시보건환경연구원 동물위생시험소;대전광역시보건환경연구원 동물위생시험소;

Received: April 26, 2021; Revised: June 19, 2021; Accepted: June 19, 2021

Isolation and identification of tick-borne pathogens in hard ticks collected in Daejeon

Han, So-young;Sung, Sun-hye;Seo, Jin-woo;Kim, Jong-ho;Lee, Seok-ju;Yoo, Sang-sik;

Daejeon Institute of Health and Environment Division of Animal Health;Daejeon Institute of Health and Environment Division of Animal Health;Daejeon Institute of Health and Environment Division of Animal Health;Daejeon Institute of Health and Environment Division of Animal Health;Daejeon Institute of Health and Environment Division of Animal Health;Daejeon Institute of Health and Environment Division of Animal Health;

Correspondence to:대전광역시보건환경연구원 동물위생시험소(Daejeon Institute of Health and Environment Division of Animal Health)

Received: April 26, 2021; Revised: June 19, 2021; Accepted: June 19, 2021

Abstract

In this study, a total of 9,449 hard ticks were collected once a month from April to October 2020 from a neighborhood park in Daejeon by flagging & dragging method and CO2 manned trap method. The collected ticks were classified according to the Yamagutsi search table using a stereoscopic microscope and molecular biological analysis of four pathogens (SFTSV, Anaplasma spp., Ehrlichia spp., Borrellia spp.). As a result of the study, Haemaphysalis longicornis were collected the most in all areas of the five boroughs at a rate of 82 to 96 percent, while adults were collected the most in May to July, nymphs were collected the most in April to June, and larvae from August to October at a rate of 78 percent to 98 percent. In pathogens, three cases of SFTSV were detected, showing a minimum infection rate (MIR) of 0.46%, while Anaplasma spp. and Ehrlichia spp. were detected one each, with 0.15% and Borrelia spp. with a minimum infection rate of 0.46%. The detected SFTSV showed 99.9% homogeneity with the KF781490 detected in Cheongwon-gun, Chungbuk Province, Anaplasma spp. showed 99.0% homogeneity with JN990105 detected in China, and Erhlichia spp. showed 98.9% genetic similarity with U96436 separated from the U.S. In this study, the distribution status and pathogen infection rate of the hard ticks in the Daejeon area are analyzed and provided as basic data for the prevention of the hard tick-borne infectious disease.

Keywords: Hard tick,SFTS,Anaplasma,Borrelia,Phylogenetic tree,

References

  1. 국가가축방역통합시스템. 법정가축전염병 발생현황. Available from: http://www.kahis.go.kr/home/lkntscrinfo/selectLkntsOccrrncList.do. Cited [2020.06.29.].
  2. 기상청, 기상자료제공포털(http://data.kma.go.kr), 대전, 2020.
  3. 서재화, 임재원, 강동호, 김흥철. 질병 매개 참진드기류의 분류와 발생현황(I). 국립생물자원관. 2015.
  4. 질병관리본부, 감염병포털, 진드기 매개감염병 관리지침. 발간등록번호 11-3152159-000823-10, pp. 66-78
  5. Wodecka et al, 2007. Significance of red deer in the ecology of Borrelia burgdorferi sensu lato, Wiad Parazytol 53: 231-237.
  6. CDC, 2017. DPDx. https://www.cdc.gov/dpdx/ticks/index.html (accessed on 31 December, 2017).
  7. Chae JB, Kim TH, Jung JH, 2017. Prevalence of severe fever with thrombocytopenia syndrome virus among ticks surveyed at Mt. Gwanak, Korea, Korean J Vet 57(3): 169- 173.
    CrossRef
  8. Chae MB, Lee JY, Kwak YG, Park SH, Lim HJ, Park SW, Chung MH, Kim MK, Kang JS, 2002. Prevalence of antibodies to Bartonella henselae and BArtonella quintana in Korean patients with Lymphadenopathy. Korean J Infect Dis 34(5): 305-310.
  9. Chong ST, Kim HC, Lee IY, Kollars TM Jr, Sancho AR, Sames WJ, Chae JS, Klein TA, 2013. Seasonal distribution of ticks in four habitats near the demilitarized zone, Gyeong gi-do(Province). Republic of Korea, J Parasitol 51: 319-325.
    Pubmed KoreaMed CrossRef
  10. Gai ZT, Zhang Y, Liang MF, Jin C, Zhang S, Zhu CB Bian PF, 2012. Clinical progress and risk factors for death in severe fever with thrombocytopenia syndrome patients. J Infect Dis 206: 1095-1102.
    Pubmed CrossRef
  11. Han SY, Lee SJ, Joung EY, Choi KS, Woo IS, Kim JH, Yoo SS, 2020. Investigation into Prevalence of zoonotic infections of companion animals in Daejeon, The report of Daejeon Institute of Health and Environment.
  12. Hwang C.Y., 2011, Ehrlichia and Borrelia spp. Infection in German shephered Dogs in Korea, J Vet Clin 28(2): 204-211.
  13. Ismail N, Bloch KC. McBride JW. 2010. Human Ehrlichiosis and An aplasmosis. Clin Lab Med 30: 261-292.
    Pubmed KoreaMed CrossRef
  14. Kang JG, Kim HC, 2013. Molecular detection of Anaplasma, Bartonella, and Borrelia species in Ticks collected from migratory birds from Hong-do island, Republic of Korea, Vector-Borned and Zoonotic Diseases 13: 1-11.
    Pubmed CrossRef
  15. KCDC, 2014. Survey on anaplasmosis and ehrlichiosis from SFTS suspicious patients in South Korea. Public Health Weekly Report, Korea Centers for Disease Control and Prevention (KCDC) 7: 979-982.
  16. KCDC, Disease Web Statistics System. Korea Centers for Disease Control and Prevention (KCDC), 2020, https://www.cdc.go.kr/npt/biz/npp/nppMain.do/ (accessed 29 June 2020).
  17. Kim CM, Kim JK, Yi YH, Lee MJ, Cho MR, Devendra H, Terry A. Klein, Kim HC, Song JW, Chong ST, Monica L. O’Guinn, 2005. Detection of Bartonella species from ticks, mites and small mammals in Korea. J Vet Sci 6(4): 327-334.
    Pubmed CrossRef
  18. Kim HC, Han SH, Chong ST, Klein TA, Choi CY, Nam HY, Chae HY, Lee H, 2011. Ticks collected from Selected Mammalian Hosts surveyed in the Republic of Korea during2008-2009. 2011. Korean J Parasitol 49: 331-335.
    Pubmed KoreaMed CrossRef
  19. Kim JB, Song HW, Park SU, Park SW, Ahn JH, Eom YB, Kim YM, 1998. Detection of Borrelia burgdorferi and Ehrlichiosis agent in ticks collected in Korea using polymerase chain reaction. Korean J Biomed 4(2): 113-120.
  20. Kim KH, Oh MD, 2014. Severe Fever with Thrombocytopenia Syndrome. Korean journal of Medicine 86: 271-274.
    CrossRef
  21. Kim KH, Yi J, Kim G. et al, 2012. Severe fever with thrombocytopenia syndrome. South Korea. Emerg Infect Dis 19: 1892-1894.
    CrossRef
  22. Kim KH, Yi J, Oh WS, Kim NH, Choi SJ, Choe PG, Kim NJ, Lee JK, Oh MD. 2014. Human graulocytic anaplasmosis, South Korea, Emerg Infec Dis 20: 1708-1711.
    Pubmed KoreaMed CrossRef
  23. Lee SO, Na DK, Kim CM, Lee YH, Cho YH, Park JH, Lee JH, Eo SK, Terry A. Klein, Chae JS, 2005. Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis bassed on 16S rRNA gene. J Vet Sci 6(2): 151-155.
    Pubmed CrossRef
  24. Moon S, Gwack J, Hwang KJ, Kwon D, Kim S, Noh Y, Roh J, Shin E-h, Jeong K, Seok W, 2013. Autochthonous lyme borreliosis in humans and ticks in Korea. Osong Public Health Res Perspect 4: 52-56.
    Pubmed KoreaMed CrossRef
  25. Noh BE, 2019. Geographical distribution and relative abundance of hard ticks in the Republic of Korea. Graduate School, Kosin University, doctoral dissertation. http://www.riss.kr/link?id=T15342418 (accessed 29 June 2020)
  26. Noh KW, Kim YJ, Ryu NS, Park JH, Kim YR, Na MS, 2017, Molecular detection for tick-borne pathogens in hard ticks collected in Jeollabukdo. 2017. The report of Jeollabukdo Animal health Division.
  27. Picken RN, Strle F, Picken MM, Ruzic-Sabljic E, Maraspin V, Lotric-Furlan S, Cimperman J., 1998. Identification of three species of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto, B. garinii, and B. afzelii) among isolates from acrodermatitis chronica atrophicans lesions. J Invest Dermatol 110: 211-214.
    Pubmed CrossRef
  28. Qin XR, Han FJ, Luo LM, et al. 2018. Anaplasma species detected in Haemaphysalis longicornis tick from China. Tick and Tick-borne Disease 9: 840-843.
    Pubmed CrossRef
  29. Sachar DS. 2000. Ehrlichia chaffeensis infection in an active duty soldier stationed in Korea. Medical Surveillance Monthly Report 6: 9-11.
  30. Shin S. 2013. Severe fever with thrombocytopenia syndrome virus. J Korean Vet Med Assoc 49: 359-364.
  31. Shinje Moon, Jin Gwack, Kyu Jam Hwang, Donghyuk Kwon, Suyeon Kim, Yoonatae Noh, Jongyul Roh, E-hyun Shin, Kungjin Jeong, Wonseok seok, Seung-Ki Youn, 2013. Autochthonous Lyme Borreliosis in Humans and Ticks in Korea. Osong Public Health Res Perspect 4(1): 52-56.
    Pubmed KoreaMed CrossRef
  32. Steere AC. 1989. Lyme disease. N. Engl. J. Med. 321: 586-596.
    Pubmed CrossRef
  33. Suh JH, Lim JW, Kang DH, Kim HC. 2015. Taxonomy and Occurrences of Disease Mediating Ticks. NIBR NO. 201501110.
  34. Takahashi T, Maeda K, Suzuki T, Ishido A, Shigeoka T, Tominaga T, 2014. The first identification and retrospective study of severe fever with thrombocytopenia syndrome in Japan. J Infect Dis 209: 816-827.
    Pubmed KoreaMed CrossRef
  35. Yu XJ, Liang MF, Zhang SY, 2011. Fever with thrombocytopenia associated with a novel bunyavirus in China. N Engl J Med 364: 1523-1532.
  36. Yun SM, Lee YJ, Choi WY, Kim HC, Chong ST, Chang KS, Jordan M. Coburn, Terry A. Klein, Lee WJ, 2016. Molecular detection of severe fever with thrombocytopenia syndromeand tick-borne encephalitis viruses in ixodid ticks collected from vegetation. Ticks and Tick- borne Diseases 7: 970-978.
    Pubmed CrossRef
KJVS
Dec 30, 2023 Vol.46 No.4, pp. 255~368

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