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Korean J. Vet. Serv. 2021; 44(1): 21-26
Published online March 30, 2021
https://doi.org/10.7853/kjvs.2021.44.1.21
© The Korean Socitety of Veterinary Service
개와 고양이에서 분리한 Pseudomonas aeruginosa의 항생제 내성
조재근;김정미;김경희;임현숙;양창렬;
대구광역시보건환경연구원;대구광역시보건환경연구원;대구광역시보건환경연구원;대구광역시보건환경연구원;대구광역시보건환경연구원;
Antimicrobial resistance of Pseudomonas aeruginosa isolated from dogs and cats
Correspondence to : 대구광역시보건환경연구원(Health & Enviornmental Research Institute of Daegu)
The aim of this study was to investigate the antimicrobial resistance among Pseudomonas (P.) aeruginosa isolated from dogs and cats. A total of 45 (6.2%) P. aeruginosa was isolated from 710 dogs and 21 cats with clinical signs. Resistance to one or more of the antimicrobials tested was observed in 26 (57.8%) P. aeruginosa. Resistance to cefepime was the most frequent (44.4%), followed by ofloxacin (22.2%), levofloxacin (17.8%), norfloxacin (8.9%), ciprofloxacin (6.7%), ceftazidime, aztreonam, colistin, polymixin B and gentamicin (4.4%, respectively), while resistance to piperacillin/tazobactam, imipenem, tobramycin and amikacin was 2.2%, respectively. All isolates were susceptibility to doripenem and meropenem. Antimicrobial susceptibility testing should be a crucial step in selection of appropriate antimicrobial therapy in veterinary medicine. Also, the prudent use of antimicrobials and continuous monitoring for companion animals are required.
Keywords Dogs,Cats,Pseudomonas aeruginosa,Antimicrobial resistance,
- 농림축산식품부. 2016. 축산분야(반려동물 포함) 항생제 내성 관리 계획.
- Arais LR, Barbosa AV, Carvalho CA, Cerqueira AM. 2016. Antimicrobial resistance, integron carriage, and
gyrA andgyrB mutations inPseudomonas aeruginosa isolated from dogs with otitis externa and pyoderma in Brazil. Vet Dermatol 27: 113-7e31.
- Bourély C, Cazeau G, Jarrige N, Leblond A, Madec JY, Haenni M, Gay E. 2019. Antimicrobial resistance patterns of bacteria isolated from dogs with otitis. Vet Microbiol 235: 280-284.
- Chamberland S, L'Ecuyer J, Lessard C, Bernier M, Provencher P, Bergeron MG. 1992. Antibiotic susceptibility profiles of 941 gram-negative bacteria isolated from septicemic patients throughout Canada. The canadian study group. Clin Infect Dis 15: 615-628.
- Chastre J, Trouillet JL. 2000. Problem pathogens (
Pseudomonas aeruginosa andAcinetobacter ). Semin Respir Infect 15: 287-298.
- Cho JK, Kim JH, Kim JM, Park CK, Kim KS. 2013. Antimicrobial resistance and distribution of resistance gene in
Enterobacteriaceae andPseudomonas aeruginosa isolated from dogs and cats. Korean J Vet Res 36: 171-180.
- CLSI. Clinical and Laboratory Standards Institute. 2017. Performance standards for antimicrobial susceptibility testing; 27th ed. CLSI supplement M100. Wayne, PA, USA.
- Eliasi UL, Sebola D, Oguttu JW, Qekwana DN. 2020. Antimicrobial resistance patterns of
Pseudomonas aeruginosa isolated from canine clinical cases at a veterinary academic hospital in South Africa. J S Afr Vet Assoc 22; e1-e6. 
- Giguere S, Prescott JF, Dowling PM. 2013: Antimicrobial Therapy in Veterinary Medicine. 5th Edition, Wiley Blackwell.
- Harada K, Arima S, Niina A, Kataoka Y, Takahashi T. 2012. Characterization of
Pseudomonas aeruginosa isolates from dogs and cats in Japan: current status of antimicrobial resistance and prevailing resistance mechanisms. Microbiol Immunol 56: 123-127.
- Hariharan H, Coles M, Poole D, Lund L, Page R. 2006. Update on antimicrobial susceptibilities of bacterial isolates from canine and feline otitis externa. Can Vet J 47: 253-255.
- Lin D, Foley SL, Qi Y, Han J, Ji C, Li R, Wu C, Shen J, Wang Y. 2012. Characterization of antimicrobial resistance of
Pseudomonas aeruginosa isolated from canine infections. J Appl Microbiol 113: 16-23.
- Linder JA, Huang ES, Steinman MA, Gonzales R, Stafford RS. 2005. Fluoroquinolone prescribing in the United States: 1995 to 2002. Am J Med 118: 259-268.
- Livermore DM, Winstanley TG, Shannon KP. 2001: Interpretative reading: recognizing the unusual and inferring resistance mechanisms from resistance phenotypes. J Antimicrob Chemother 48(Suppl. S1): 87-102.
- Markey BF, Leonard M, Archambault A, Maguire CD. 2013. Clinical veterinary microbiology. 2nd ed., Mosby Elsevier, Edinburgh, London, New York, Oxford, Philadelphia, St. Louis, Sydney, Toronto, pp. 275-284.
- Martín Barrasa JL, Lupiola Gómez P, González Lama Z, Tejedor Junco MT. 2000. Antibacterial susceptibility patterns of
Pseudomonas strains isolated from chronic canine otitis externa. J Am Anim Hosp Assoc 43: 307-312. - Mekić S, Matanović K, Šeol B. 2011. Antimicrobial susceptibility of
Pseudomonas aeruginosa isolates from dogs with otitis externa. Vet Rec 169: 125.
- Normand EH, Gibson NR, Taylor DJ, Carmichael S. Reid SW 2000. Trends of antimicrobial resistance in bacterial isolates from a small animal referral hospital. Vet Rec 146: 151-155.
- Park SY, Bae SG, Kim JT, Oh TH. 2017. Identification and antimicrobial susceptibility of bacteria isolated from dogs with chronic otitis externa. J Vet Clin 34: 23-26.
- Park YJ, Oh JY, Park SW, Sum SU, Song WK, Chae JC, Park HM. 2020. Antimicrobial resistance and novel mutations detected in the
gyrA andparC genes ofPseudomonas aeruginosa strains isolated from companion dogs. BMC Vet Res 16: 111.
- Pedersen K, Pedersen K, Jensen H, Finster K, Jensen VF, Heuer OE. 2007. Occurrence of antimicrobial resistance in bacteria from diagnostic samples from dogs. J Antimicrob Chemother 60: 775-781.
- Perez F, Hujer AM, Hujer KM, Decker BK, Rather PN, Bonomo RA. 2007. Global challenger of multidrug-resistant
Acinetobacter baumannii . Antimicrob Agents Chemother 51: 3471-3484.
- Petersen AD, Walker RD, Bowman MM, Schott HC. 2002. Frequency of isolation and antimicrobial susceptibility patterns of
Staphylococcus intermedius andPseudomonas aeruginosa isolates from canine skin and ear samples over a 6-year period (1992-1997). J Am Anim Hosp Assoc 38: 407-413.
- Pintarić S, Krešimir Matanović K, Martinec BS. 2017. Fluoroquinolone susceptibility in
Pseudomonas aeruginosa isolates from dogs-comparing disk diffusion and microdilution methods. Veterinarski Arhiv 87: 291-300.
- Rubin J, Walker RD, Blickenstaff K, Bodeis-Jones S, Zhao S. 2008. Antimicrobial resistance and genetic characterization of fluoroquinolone resistance of
Pseudomonas aeruginosa isolated from canine infections. Vet Microbiol 131: 164-172.
- Seol B, Naglić T, Madić J, Bedeković M. 2002. In Vitro Antimicrobial Susceptibility of 183
Pseudomonas aeruginosa strains isolated from dogs to selected antipseudomonal agents. J Vet Med B Infect Dis Vet Public Health 49: 188-192.
- Wildermuth BE, Griffin CE, Rosenkrantz WS, Boord MJ. 2007. Susceptibility of
Pseudomonas isolates from the ears and skin of dogs to enrofloxacin, marbofloxacin and ciprofloxacin. J Am Anim Hosp Assoc 43: 337-341.
- Yukawa S, Tsuyuki Y, Sato T, Fukuda A, Usui M, Tamura Y. 2017. Antimicrobial resistance of
Pseudomonas aeruginosa isolated from dogs and cats in primary veterinary hospitals in Japan. Jpn J Infect Dis 70: 461-463.
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Research Article
Korean J. Vet. Serv. 2021; 44(1): 21-26
Published online March 30, 2021 https://doi.org/10.7853/kjvs.2021.44.1.21
Copyright © The Korean Socitety of Veterinary Service.
개와 고양이에서 분리한 Pseudomonas aeruginosa의 항생제 내성
조재근;김정미;김경희;임현숙;양창렬;
대구광역시보건환경연구원;대구광역시보건환경연구원;대구광역시보건환경연구원;대구광역시보건환경연구원;대구광역시보건환경연구원;
Antimicrobial resistance of Pseudomonas aeruginosa isolated from dogs and cats
Cho, Jae-Keun;Kim, Jeong-Mi;Kim, Kyung-Hee;Lim, Hyun-Suk;Yang, Chang-Ryoul;
Health & Enviornmental Research Institute of Daegu;Health & Enviornmental Research Institute of Daegu;Health & Enviornmental Research Institute of Daegu;Health & Enviornmental Research Institute of Daegu;Health & Enviornmental Research Institute of Daegu;
Correspondence to:대구광역시보건환경연구원(Health & Enviornmental Research Institute of Daegu)
Abstract
The aim of this study was to investigate the antimicrobial resistance among Pseudomonas (P.) aeruginosa isolated from dogs and cats. A total of 45 (6.2%) P. aeruginosa was isolated from 710 dogs and 21 cats with clinical signs. Resistance to one or more of the antimicrobials tested was observed in 26 (57.8%) P. aeruginosa. Resistance to cefepime was the most frequent (44.4%), followed by ofloxacin (22.2%), levofloxacin (17.8%), norfloxacin (8.9%), ciprofloxacin (6.7%), ceftazidime, aztreonam, colistin, polymixin B and gentamicin (4.4%, respectively), while resistance to piperacillin/tazobactam, imipenem, tobramycin and amikacin was 2.2%, respectively. All isolates were susceptibility to doripenem and meropenem. Antimicrobial susceptibility testing should be a crucial step in selection of appropriate antimicrobial therapy in veterinary medicine. Also, the prudent use of antimicrobials and continuous monitoring for companion animals are required.
Keywords: Dogs,Cats,Pseudomonas aeruginosa,Antimicrobial resistance,
References
- 농림축산식품부. 2016. 축산분야(반려동물 포함) 항생제 내성 관리 계획.
- Arais LR, Barbosa AV, Carvalho CA, Cerqueira AM. 2016. Antimicrobial resistance, integron carriage, and
gyrA andgyrB mutations inPseudomonas aeruginosa isolated from dogs with otitis externa and pyoderma in Brazil. Vet Dermatol 27: 113-7e31.
- Bourély C, Cazeau G, Jarrige N, Leblond A, Madec JY, Haenni M, Gay E. 2019. Antimicrobial resistance patterns of bacteria isolated from dogs with otitis. Vet Microbiol 235: 280-284.
- Chamberland S, L'Ecuyer J, Lessard C, Bernier M, Provencher P, Bergeron MG. 1992. Antibiotic susceptibility profiles of 941 gram-negative bacteria isolated from septicemic patients throughout Canada. The canadian study group. Clin Infect Dis 15: 615-628.
- Chastre J, Trouillet JL. 2000. Problem pathogens (
Pseudomonas aeruginosa andAcinetobacter ). Semin Respir Infect 15: 287-298.
- Cho JK, Kim JH, Kim JM, Park CK, Kim KS. 2013. Antimicrobial resistance and distribution of resistance gene in
Enterobacteriaceae andPseudomonas aeruginosa isolated from dogs and cats. Korean J Vet Res 36: 171-180.
- CLSI. Clinical and Laboratory Standards Institute. 2017. Performance standards for antimicrobial susceptibility testing; 27th ed. CLSI supplement M100. Wayne, PA, USA.
- Eliasi UL, Sebola D, Oguttu JW, Qekwana DN. 2020. Antimicrobial resistance patterns of
Pseudomonas aeruginosa isolated from canine clinical cases at a veterinary academic hospital in South Africa. J S Afr Vet Assoc 22; e1-e6.
- Giguere S, Prescott JF, Dowling PM. 2013: Antimicrobial Therapy in Veterinary Medicine. 5th Edition, Wiley Blackwell.
- Harada K, Arima S, Niina A, Kataoka Y, Takahashi T. 2012. Characterization of
Pseudomonas aeruginosa isolates from dogs and cats in Japan: current status of antimicrobial resistance and prevailing resistance mechanisms. Microbiol Immunol 56: 123-127.
- Hariharan H, Coles M, Poole D, Lund L, Page R. 2006. Update on antimicrobial susceptibilities of bacterial isolates from canine and feline otitis externa. Can Vet J 47: 253-255.
- Lin D, Foley SL, Qi Y, Han J, Ji C, Li R, Wu C, Shen J, Wang Y. 2012. Characterization of antimicrobial resistance of
Pseudomonas aeruginosa isolated from canine infections. J Appl Microbiol 113: 16-23.
- Linder JA, Huang ES, Steinman MA, Gonzales R, Stafford RS. 2005. Fluoroquinolone prescribing in the United States: 1995 to 2002. Am J Med 118: 259-268.
- Livermore DM, Winstanley TG, Shannon KP. 2001: Interpretative reading: recognizing the unusual and inferring resistance mechanisms from resistance phenotypes. J Antimicrob Chemother 48(Suppl. S1): 87-102.
- Markey BF, Leonard M, Archambault A, Maguire CD. 2013. Clinical veterinary microbiology. 2nd ed., Mosby Elsevier, Edinburgh, London, New York, Oxford, Philadelphia, St. Louis, Sydney, Toronto, pp. 275-284.
- Martín Barrasa JL, Lupiola Gómez P, González Lama Z, Tejedor Junco MT. 2000. Antibacterial susceptibility patterns of
Pseudomonas strains isolated from chronic canine otitis externa. J Am Anim Hosp Assoc 43: 307-312. - Mekić S, Matanović K, Šeol B. 2011. Antimicrobial susceptibility of
Pseudomonas aeruginosa isolates from dogs with otitis externa. Vet Rec 169: 125.
- Normand EH, Gibson NR, Taylor DJ, Carmichael S. Reid SW 2000. Trends of antimicrobial resistance in bacterial isolates from a small animal referral hospital. Vet Rec 146: 151-155.
- Park SY, Bae SG, Kim JT, Oh TH. 2017. Identification and antimicrobial susceptibility of bacteria isolated from dogs with chronic otitis externa. J Vet Clin 34: 23-26.
- Park YJ, Oh JY, Park SW, Sum SU, Song WK, Chae JC, Park HM. 2020. Antimicrobial resistance and novel mutations detected in the
gyrA andparC genes ofPseudomonas aeruginosa strains isolated from companion dogs. BMC Vet Res 16: 111.
- Pedersen K, Pedersen K, Jensen H, Finster K, Jensen VF, Heuer OE. 2007. Occurrence of antimicrobial resistance in bacteria from diagnostic samples from dogs. J Antimicrob Chemother 60: 775-781.
- Perez F, Hujer AM, Hujer KM, Decker BK, Rather PN, Bonomo RA. 2007. Global challenger of multidrug-resistant
Acinetobacter baumannii . Antimicrob Agents Chemother 51: 3471-3484.
- Petersen AD, Walker RD, Bowman MM, Schott HC. 2002. Frequency of isolation and antimicrobial susceptibility patterns of
Staphylococcus intermedius andPseudomonas aeruginosa isolates from canine skin and ear samples over a 6-year period (1992-1997). J Am Anim Hosp Assoc 38: 407-413.
- Pintarić S, Krešimir Matanović K, Martinec BS. 2017. Fluoroquinolone susceptibility in
Pseudomonas aeruginosa isolates from dogs-comparing disk diffusion and microdilution methods. Veterinarski Arhiv 87: 291-300.
- Rubin J, Walker RD, Blickenstaff K, Bodeis-Jones S, Zhao S. 2008. Antimicrobial resistance and genetic characterization of fluoroquinolone resistance of
Pseudomonas aeruginosa isolated from canine infections. Vet Microbiol 131: 164-172.
- Seol B, Naglić T, Madić J, Bedeković M. 2002. In Vitro Antimicrobial Susceptibility of 183
Pseudomonas aeruginosa strains isolated from dogs to selected antipseudomonal agents. J Vet Med B Infect Dis Vet Public Health 49: 188-192.
- Wildermuth BE, Griffin CE, Rosenkrantz WS, Boord MJ. 2007. Susceptibility of
Pseudomonas isolates from the ears and skin of dogs to enrofloxacin, marbofloxacin and ciprofloxacin. J Am Anim Hosp Assoc 43: 337-341.
- Yukawa S, Tsuyuki Y, Sato T, Fukuda A, Usui M, Tamura Y. 2017. Antimicrobial resistance of
Pseudomonas aeruginosa isolated from dogs and cats in primary veterinary hospitals in Japan. Jpn J Infect Dis 70: 461-463.
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