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Korean J. Vet. Serv. 2008; 31(2): 195-205
Published online June 30, 2008
© The Korean Socitety of Veterinary Service
소와 돼지도체에서 Yersinia enterocolitica의 분리 및 특성
채희선;김주영;김지은;양윤모;진경선;신방우;김선홍;이정학;
서울특별시 보건환경연구원;서울특별시 보건환경연구원;서울특별시 보건환경연구원;서울특별시 보건환경연구원;서울특별시 보건환경연구원;서울특별시 보건환경연구원;서울특별시 보건환경연구원;서울특별시 보건환경연구원;
Characteristics of Yersinia enterocolitica isolates from beef and pork carcass
Correspondence to : 서울특별시 보건환경연구원(Seoul Metropolitan Government Research Institute of Public Health & Environment)
Yersinia enterocolitica is a zoonotic agent, and to cause food poisoning. This study was carried out to get some basic information for the control of Yersinia infection. A total of 1,680 samples were collected from beef and pork carcasses from January 2006 to December 2007 in Seoul. The isolation rate was higher in pork carcass than in beef carcass. Five (0.59%) Yersinia enterocolitica were isolated from the 840 of beef carcasses, and eighteen(2.14%) were isolated from the 840 of pork carcasses. Among 23 strains, 22 were classified into biotype 1A, and one was biotype 6. In serotyping of Y enterocolitica isolates, 21 strains were untypable (UT), and 2 were O5 and O8 respectively. In PCR, Ail gene was not detected in all of 23 strains that determined non-pathogenic. In antimicrobial susceptibility test, twelve strains (52.2%) of 23 isolates showed the multi -resistant patterns with over 3 drugs. PFGE was performed after the genomic DNA of twenty three isolates, which was digested with Xba I. the 23 isolates showed 12 (
Keywords Yersinia enterocolitica,Ail gene,Serotype,PFGE,
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Research Article
Korean J. Vet. Serv. 2008; 31(2): 195-205
Published online June 30, 2008
Copyright © The Korean Socitety of Veterinary Service.
소와 돼지도체에서 Yersinia enterocolitica의 분리 및 특성
채희선;김주영;김지은;양윤모;진경선;신방우;김선홍;이정학;
서울특별시 보건환경연구원;서울특별시 보건환경연구원;서울특별시 보건환경연구원;서울특별시 보건환경연구원;서울특별시 보건환경연구원;서울특별시 보건환경연구원;서울특별시 보건환경연구원;서울특별시 보건환경연구원;
Characteristics of Yersinia enterocolitica isolates from beef and pork carcass
Chae, Hee-Sun;Kim, Joo-Young;Kim, Jee-Eun;Yang, Yun-Mo;Jin, Kyung-Sun;Shin, Bang-Woo;Kim, Sun-Heung;Lee, Jung-Hark;
Seoul Metropolitan Government Research Institute of Public Health & Environment;Seoul Metropolitan Government Research Institute of Public Health & Environment;Seoul Metropolitan Government Research Institute of Public Health & Environment;Seoul Metropolitan Government Research Institute of Public Health & Environment;Seoul Metropolitan Government Research Institute of Public Health & Environment;Seoul Metropolitan Government Research Institute of Public Health & Environment;Seoul Metropolitan Government Research Institute of Public Health & Environment;Seoul Metropolitan Government Research Institute of Public Health & Environment;
Correspondence to:서울특별시 보건환경연구원(Seoul Metropolitan Government Research Institute of Public Health & Environment)
Abstract
Yersinia enterocolitica is a zoonotic agent, and to cause food poisoning. This study was carried out to get some basic information for the control of Yersinia infection. A total of 1,680 samples were collected from beef and pork carcasses from January 2006 to December 2007 in Seoul. The isolation rate was higher in pork carcass than in beef carcass. Five (0.59%) Yersinia enterocolitica were isolated from the 840 of beef carcasses, and eighteen(2.14%) were isolated from the 840 of pork carcasses. Among 23 strains, 22 were classified into biotype 1A, and one was biotype 6. In serotyping of Y enterocolitica isolates, 21 strains were untypable (UT), and 2 were O5 and O8 respectively. In PCR, Ail gene was not detected in all of 23 strains that determined non-pathogenic. In antimicrobial susceptibility test, twelve strains (52.2%) of 23 isolates showed the multi -resistant patterns with over 3 drugs. PFGE was performed after the genomic DNA of twenty three isolates, which was digested with Xba I. the 23 isolates showed 12 (
Keywords: Yersinia enterocolitica,Ail gene,Serotype,PFGE,
