Korean Journal of
Veterinary Service

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Abstract : Contents of nitrites was tested in 2,290 meat products during 2000-2003, in Seoul by Diazoa method. It was detected over 40 ppm NO$_2$$^$-/ contents in 20 hams, 7 sausages, one bacon, and one crushed meat product respectively. Also, over 20ppm nitrites was 21.8%(240/1,103) in hams, 20.7%(122/589) in sausages, 6.8%(14/205) in crushed meats, and in 6.0%(5/83) bacons respectively. In case of average contents and contents range, 0.012 g/kg, ND-0.116 g/kg in hams, 0.012 g/kg, ND-0.066 g/kg in sausages, 0.010 g/kg, 0.001-0.089 g/kg in bacons, and 0.006 g/kg, ND-0.040 g/kg in crushed meats etc. Specially, in sausages, it was increased continually by years, in not only average nitrites contents but also their contents range, also, in case of bacons, increased continually by years on only average nitrites contents. According to results, the NO$_2$$^$-/ contents monitoring for the processed meat products must be reinforced to supply safety food for the citizens.

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Abstract : This experiment was executed to control effectively endemic disease of swine farms in Incheon metropolitan city. Mainly using PigMon program which had been developed by the College of Veterinary Medicine in the University of Minnesota(USA), we examined lesions of gastric ulcer and interstitial nephritis additionally. 446 heads of pigs shipped from 5 farms in Incheon to a slaughterhouse from June in 2003 to May in 2004 were examined. Infection rates by farms were obtained as follows; 1. Pneumonia was varied from 34.6% to 74.1% and pneumonic score was 1.47∼7.06. As for atrophic rhinitis, four farms were 100% and one farm was 89.5% and rhinitis score was 1.3∼3.2. 2. The infection rate of pericarditis and peritonitis was 1.0∼3.9% and liver white spots, papular dermatitis were observed in pigs of 9.8∼29.7%, 16.7∼51.4% respectively. 3. The outbreak rates of interstitial nephritis, lesions of ileal thickening and gastric ulcer were 15.4∼24.1%, 7.7∼13.5%, and 62.7%∼86.2% respectively.

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Abstract : This study was attempted to survey on the prevalence of canine heartworm(Dirofilaria immitis) infections among 206 dogs (male 92, female 114) in Daejeon area from March to December 2003. Blood samples taken from dogs were examined for the presence of D immitis microfilaria by the modified Knott's test and an antigen test( $AGEN^{TM}$ canine heartworm). The result obtained from this experiment were summarized as follows; 1. Eighteen(8.7%) of the 206 examined dogs were microfilaria positive, while twenty five dogs(12.1%) were antigen test positive. 2. The infection rates of heartworm in dogs at the age of <2, 2∼4 and $geq$5 were 3.8%, 18% and 25%, respectively. 3. The infection rates of heartworm in dogs by housing of indoor and outdoor were 3.3% and 36.4%, respectively. 4. The regional infection rates were Dong-gu(13/29, 44.8%), Seo-gu(7/92, 7.6%), Yuseong-gu(2/33, 6.1%), Jung-gu(1/17, 5.9%) and Daedeok-gu(2/35, 5.7%). 5. The seasonal infection rates were summer(June∼August, 16.7%), autumn (September∼October, 14%), spring(March∼May, 9.4%) and winter(November∼December. 7.7%).7.7%).

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Abstract : This study was carried out to investigate the prevalence of pocine circovirus type 2(PCV2) infection and post-weaning multi systemic wasting syndrome(PMWS) of swine in Gyeongbuk province from June to December in 2003. Gyeongbuk Veterinary Service Laboratory had tested PCV1 and PCV2 by the PCR method against 210 infected pigs of 105 farms, which were requested diagnosis of disease and investigate compositive infections with bacterial disease. The following results were obtained; 1. Among 210 pigs, The pigs infected with PCV1 were 134(63.8%) heads and with PCV2 were 143(68.1%) heads. 2. Using PCR, the rate of PCV1 infection of 105 farms which were requested of diagnosis of disease is positive in 75(71.4%) farms and 78(74.3%) farms were positive in PCV2, and compositive infections of PCV1 and PCV2 were 75(71.4%) farms. 3. According to age of requested pig, the frequency of PCV infection rate was following, the pig under 4 weeks of age were 28.6% in PCV1 and PCV2, respectively, and the pig of 4-7 weeks of age were 58.6% in PCV1 and PCV2, respectively. The pig of 8-11 weeks of age were 73.1% in PCVl and 74.6% in PCV2, the pig of 12-18 weeks of age were 80.9% in PCV1 and 88.1% in PCV2. 4. In the distribution of compositive infection with bacterial disease related to PCV2 among requested samples, PCV2 infection was 39.7% in 78 cases, two kinds of compositive infection was 38.5%, three kinds of compositive infection was 18.1% and four kinds of complex infection was 3.9%.

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Abstract : The objective of present study was to assess the diagnostic value of the concentration of haptoglobin, one of a series of acute phase proteins that is found in the blood of both humans and animals. A total of 79 random horse sera(10 dam and 69 foals) with clinically normal were analyzed by ELISA. The mean serum concentrations of haptoglobin of dam and foals were 1.67${pm}$0.52mg/ml(range 1.08∼2.12 mg/ml), 1.65${pm}$0.50 mg/ml(range 0.50∼2.66 mg/ml), respectively. These results may provide basic information for developing a useful marker system to monitor infections early in horses.

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Abstract : Bacteria isolated from nasal cavity of 50 Thoroughbred horses with respiratory tract infection were examined. There were isolated Pseudomonas aeruginosa(33.5%), Escherichia coli(10.2%), Pseudomonas spp(7.6%), Klebsiella oxytoca(5.9%), Streptococcus equi subsp zooepidemious(6.2%), Klebsiella pneumoniae(3.4%), Acinetobacter spp(5.5%) and coagulase negative staphylococcus(2.1%). The majority of isolates were highly susceptible to amikacin, amoxicillin, aztreonam, cefotaxime, cefepime, cefotetan, ceftazidime, cefuroxime, chloramphenicol, ciprofloxacin, clindamycin, erythromycin, gentamicin, imipenem, tetracyclin and vancomycin. These results can provide basic information for the treatments of respiratory tract infections in Thoroughbred horses.

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Abstract : A dairy farm that has been suffered continuously(more than 2 years) from brucellosis in Korea in spite of repeated legal test-and-slaughter was investigated the main source of infection in the farm. All cattle(22 milking cows, 44 heifers, 60 calves, 8 bull), dogs(3 mixed breed), feces from wild birds(3 samples), drinking water(3 sites), and soil in the paddocks(14 sites) inside the farm were examined with serological and/or bacteriological methods including specific DNA detection with PCR method. Brucella spp in the milk and blood were detected in 12/22 and 5/22 milking cows, respectively, although all of them were negative with conventional tube agglutination test. The number of serologically positive heifer was 15(15/44), but the isolation of Brucella spp was succeeded in the only 11(11/15) of them. Brucella were detected in vagina 1(1/11) and nasal(3/12) excretion in serologically positive heifers. All the three dogs were serologically positive, and Brucella spp were isolated from their blood. However, Brucella spp were not detected in the drinking water, soil in the paddocks, nor the feces of wild birds. The results suggest that milking cow secrete Brucella spp through milk, genital tract and nasal cavity, which are the major source of infection in this farm, The main infection route of Brucella spp is contact to contact with Brucella spp excreting animals rather than environmental contamination. The animals, living together with infected cow such as dogs, are the readily susceptible and are required to be examined for Brucella spp.

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Abstract : In order to monitor the parasites, 83 fecal samples were taken from monkeys such as Cercopithecus mitis(6), Macaca fuscata(32), Saimiri sciurea(27), Macaca nigra(5), Papio hamadrysa(8), and Macaca mulatta(5) in Jeonbuk area. Isolation and Identification of the parasites from the feces were determined by the fecal examination using the floatation and microscopical examination, respectively. The detection rate was 21.6%, and mixed infection rate was single 18.0%(15 heads), double 2.4%(2 heads), and triple 1.2% (1 head). The isolates were identified as Strongyloides axei from 7 heads. Trichuris spp from 3 heads, Entamoeba histolytica 3 heads, Toxacaris leonina from 3 heads, and Eimeria spp from 2 heads.