Korean Journal of
Veterinary Service

Alignment of amino N-terminal 1-234 amino acid of spike (S) proteins between the reference strains and twenty PEDV field strains in Jeonbuk. The dashes (−) indicate the deleted sequences. Insertions and deletions within PEDV isolates are indicated by boxes (Red box indicate the inserted amino acids compared with SM-98 and CV777. Blue box indicates the deleted amino acids compared with SM-98 or CV777).|@|~(^,^)~|@|Phylogenetic analysis based on the nucleotide sequence corresponding to the full-length S gene of PEDV strains. Sequencing alignments were performed using the Clustal W program, and the phylogenetic tree was constructed from the aligned nucleotide sequences by using the neighbor-joining method. The bootstrap values were determined from 1,000 replicates of original data. The scale bars indicate the number of 0.050 inferred substitutions per site. Putative similar regions of S gene of other distantly related coronavirus, transmissible gastroenteritis virus (TGEV), was also included in this study.|@|~(^,^)~|@|Comparison of the antigen epitopes between field strains and reference strains. Dots indicate the amino acids are identical to those of reference strain SM-98-1. COE is in the red box, SS2 is in the blue box, SS6 is in the green box and the against 2C10 is in the orange box. The mutated amino acids within PEDV isolates are indicated by boxes and circles. Black box indicates the mutated amino acids compared with SM-98-1.

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