Korean Journal of
Veterinary Service

The limit of detection (LOD) and standard curve of duplex real-time polymerase chain reaction (dqPCR) in the presence of serum, tissue, and saliva. (A, C, E) LOD of dqPCR for serial 10-fold dilutions of PCV4 standard DNA spiked into pig samples of serum, tissue, and saliva, respectively. Lines 6∼ 0, 10-fold serial dilutions of the PCV4 standard DNA (5 × 10⁶–10⁰ copies). (B, D, F) Standard curve for dqPCR using serial 10-fold dilutions of PCV4 standard DNA (5×10⁶–10⁰ copies) spiked into pig samples of serum, tissue, and saliva were plotted against the threshold cycle on (Ct). The coefficient of determination (R²) and the equation of the regression curve (y) were calculated using the CFX Manager Software (Bio-Rad).|@|~(^,^)~|@|Comparison of sensitivities of a duplex real-time polymerase chain reaction (dqPCR) among Zhang’s TaqMan probe-based real-time polymerase chain reaction (qPCR), Zhang’s SYBR Green-based real-time polymerase chain reaction (qPCR), and Tian’s conventional polymerase chain reaction (cPCR). The limit of detections (LOD) and standard curve of the dqPCR (A, B), LOD and melting curve of the Zhang’s SYBR Green-based qPCR (C, D), and LOD of the Zhang’s TaqMan probe-based qPCR and Tian’s cPCR (E, F). Lines 6-0, 10-fold serial dilutions of the PCV4 standard DNA (5×10⁶−10⁰ copies); NC, negative control.

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